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APC anti-mouse CD126 (IL-6Rα chain) Antibody

Pricing & Availability
Clone
D7715A7 (See other available formats)
Regulatory Status
RUO
Other Names
IL-6 Receptor α chain, IL-6R
Isotype
Rat IgG2b, κ
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Product Citations
publications
D7715A7_APC_041910
Mouse myeloma FO cells stained with D7715A7 APC
  • D7715A7_APC_041910
    Mouse myeloma FO cells stained with D7715A7 APC
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115811 25 µg $125
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115812 100 µg $296
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Description

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. The D7715A7 (15A7) antibody blocks IL-6/IL-6 receptor interactions.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
OKT4 hybridoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,5, in vivo receptor blocking1-2,4-6,8, and in vitro neutralization7. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115804) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115808).

Please note, this clone had previously reported reactivity to rat, however upon further testing it has been determined to not react with rat.

Application References

(PubMed link indicates BioLegend citation)
  1. Coulie PG, et al. 1990. Curr. Top. Microbiol. Immunol. 166:43. (Block, IP)
  2. Vink A, et al. 1990. J. Exp. Med. 172:997. (Block, IP)
  3. Sun R, et al. 2004. J. Immunol. 172:5648. (FC)
  4. McKinney WJ, et al. 1998. Am. J. Respir. Cell Mol. Biol. 18:696. (Block)
  5. Weigert C, et al. 2006. J. Biol. Chem. 281:7060. (Block, IP)
  6. Koizumi N, et al. 2007. J. Immunol. 178:1767. (Block)
  7. Walker F, et al. 2008. Blood 111:3978. (Neut) PubMed
  8. Yadav M, et al. 2008. J. Immunol. 180:2772. (Block) PubMed
  9. Sintes J, et al. 2010. J. Leukoc. Biol. 88:687. PubMed
Product Citations
  1. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  2. Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed
  3. Figueiredo ML, et al. 2020. Mol Ther Methods Clin Dev. 17:739. PubMed
  4. Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
  5. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  6. Ng S, et al. 2022. Sci Rep. 12:14087. PubMed
RRID
AB_2127937 (BioLegend Cat. No. 115811)
AB_2296238 (BioLegend Cat. No. 115812)

Antigen Details

Structure
Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution

Activated T cells and B cells, monocytes, hepatocytes, plasma cells

Function
Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand/Receptor
IL-6
Cell Type
B cells, Mesenchymal Stem Cells, Monocytes, Plasma cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taga T, et al. 1997. Annu. Rev. Immunol. 15:797.
2. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London.
3. Boulanger MJ, et al. 2003. Science 300:2101.

Gene ID
16194 View all products for this Gene ID
UniProt
View information about CD126 on UniProt.org
Go To Top Version: 2    Revision Date: 11/08/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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