APC anti-mouse CD121a (IL-1 R, Type I/p80) Antibody

Pricing & Availability
Clone
JAMA-147 (See other available formats)
Other Names
IL-1 Receptor type I, IL-1RI, p80
Isotype
Armenian Hamster IgG
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Product Citations
publications
JAMA147_APC_020110
Mouse fibroblast NIH/3T3 cells stained with JAMA-147 APC
  • JAMA147_APC_020110
    Mouse fibroblast NIH/3T3 cells stained with JAMA-147 APC
See APC spectral data
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113509 50 µg $120
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Description

CD121a is an 80 kD IL-1 receptor type I. It is a transmembrane glycoprotein and a member of the immunoglobulin superfamily expressed on T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells and neural cells. CD121a binds the mature forms of IL-1α, IL-1β, and IL-1Ra proteins. High affinity binding and signaling by the type I IL-1 receptor requires CD121a and the accessory protein, IL-1R AcP, which does not bind IL-1 alone, but forms a dimer capable of binding IL-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Extracellular domain of mouse IL-1R1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and receptor blocking in vivo1. Clone JAMA-147 prevents the binding of IL-1 to the type 1 IL-R1,2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for neutralization and functional assays (contact our custom solutions team).

For the most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 113504) or biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Rogers HK, et al. 1992. P. Natl. Acad. Sci. USA 89:1011. (Neut)
  2. Rogers HW, et al. 1994. J. Immunol. 153:2093. (Neut)
Product Citations
  1. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
Publication Library
RRID
AB_2264757 (BioLegend Cat. No. 113509)

Antigen Details

Structure
Ig superfamily, complex of CD121a and IL-1R AcP forms high affinity IL-1 binding site, 80 kD
Distribution
T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells, neural cells
Function
Innate and adaptive immune response
Ligand/Receptor
IL-1α and IL-1β
Cell Type
T cells, Thymocytes, Dendritic cells, Fibroblasts, Endothelial cells, Epithelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sims JE. 2002. Curr. Opin. Immunol. 14:117.
2. Rogers HK, et al. 1992. P. Natl. Acad. Sci. USA 89:1011.

Gene ID
16177 View all products for this Gene ID
UniProt
View information about CD121a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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