APC anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
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105811 25 µg $84
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105812 100 µg $209
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Lengefeld J, et al. 2021. Sci Adv. 7:eabk0271. PubMed
  3. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  4. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  5. Barkaway A, et al. 2021. Immunity. . PubMed
  6. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  7. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  8. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  9. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  10. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  11. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  12. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  13. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  14. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  15. Zhu H, et al. 2018. Elife. 7:. PubMed
  16. Chen CC, et al. 2020. Cancer Cell. 37:71. PubMed
  17. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  18. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
  19. Sun Y et al. 2018. Cancer cell. 34(4):643-658 . PubMed
  20. Ludin A, et al. 2012. Nat Immunol. 13:1072. PubMed
  21. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  22. Guo B, et al. 2018. Nat Med. 24:360. PubMed
  23. Gao B, et al. 2018. Aging Cell. 3. PubMed
  24. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  25. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  26. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  27. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  28. Nguyen DH, et al. 2020. Nat Cell Biol. 22:1423. PubMed
  29. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  30. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  31. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  32. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  33. Widner DB, et al. 2021. J Cachexia Sarcopenia Muscle. 12:1079. PubMed
  34. Eisele AS, et al. 2022. Elife. 11:. PubMed
  35. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  36. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  37. Plein A, et al. 2018. Nature. 562:223. PubMed
  38. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  39. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  40. Ritter M, et al. 2020. Ann Hematol. 99:2329. PubMed
  41. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  42. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  43. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  44. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  45. Dong S, et al. 2021. Nature. 591:117. PubMed
  46. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  47. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  48. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  49. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  50. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  51. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  52. Convente MR, et al. 2018. J Bone Miner Res. 33:269. PubMed
  53. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  54. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  55. Umemoto T, et al. 2008. Biochem Biophys Res Commun. 365:176. PubMed
  56. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  57. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  58. Zhu M, et al. 2015. J Immunol. 195: 4492 - 4502. PubMed
  59. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  60. Wang X, et al. 2020. Cell Res. 30:1109. PubMed
  61. Sibon D et al. 2019. Cell reports. 26(12):3246-3256 . PubMed
  62. Tsuchiya K, et al. 2019. Nat Commun. 10:2091. PubMed
  63. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  64. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  65. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  66. Li W, et al. 2021. PLoS Pathog. 17:e1009462. PubMed
  67. N. H, et al. 2017. Journal of Vascular Research. 10.1159/000464287. PubMed
  68. Gandhapudi S, et al. 2015. J Immunol. 194:3820. PubMed
  69. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  70. Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed
  71. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  72. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  73. Legrand JMD, et al. 2019. Nat Commun. 10:2278. PubMed
  74. Engblom C, et al. 2017. Science. 358:6367. PubMed
  75. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  76. Suzuki S, et al. 2021. Cell Reports. 34(7):108752. PubMed
  77. Wilson ZS, et al. 2020. Sci Rep. 10:2142. PubMed
  78. Koide S, et al. 2022. iScience. 25:103603. PubMed
  79. Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed
  80. Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed
  81. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  82. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  83. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  84. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  85. Artinger E, et al. 2013. Proc Natl Acad Sci U S A. 110:12000. PubMed
  86. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  87. Dhayade S, et al. 2020. Nutrients. 12:. PubMed
  88. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  89. Brian BF 4th et al. 2019. Elife. 8 pii: e46043. PubMed
  90. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  91. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  92. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  93. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  94. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  95. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  96. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  97. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  98. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  99. Liu J, et al. 2012. Proc Natl Acad Sci U S A. 109:10018. PubMed
  100. Collins C, et al. 2014. Proc Natl Acad Sci U S A. 111:9899. PubMed
  101. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  102. Cui Y, et al. 2020. Eur J Pharmacol. 881:173233. PubMed
  103. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  104. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  105. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
RRID
AB_313220 (BioLegend Cat. No. 105811)
AB_313221 (BioLegend Cat. No. 105812)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/28/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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