APC anti-human CD357 (GITR) Antibody

Pricing & Availability
Clone
621 (See other available formats)
Workshop
HCDM listed
Other Names
Activation-inducible TNFR, AITR, Glucocorticoid-inducible TNFR, TNFRSF18
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
621_APC_120109
Human T lymphoma cell line HUT-78 stained with 621 APC
  • 621_APC_120109
    Human T lymphoma cell line HUT-78 stained with 621 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
311609 25 tests $145
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311610 100 tests $325
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Description

GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18). GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and is involved in the development of regulatory T cells and to regulate the activity of Th1 subsets.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 311606).

Application References

(PubMed link indicates BioLegend citation)
  1. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.
  2. Zhou Z, et al. 2008. Proc Natl Acad Sci USA . 105:5465 PubMed
RRID
AB_2256064 (BioLegend Cat. No. 311609)
AB_2204247 (BioLegend Cat. No. 311610)

Antigen Details

Structure
TNFR superfamily, 234-amino acid type I transmembrane protein, 25 kD
Distribution

Activated lymphocytes

Function
Interacts with TRAF's 1-3, but not TRAF 5 or 6, mediates NF-κB activation through the TRAF2/NIK pathway
Ligand/Receptor
GITRL
Cell Type
Lymphocytes, Tregs
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.

Gene ID
8784 View all products for this Gene ID
UniProt
View information about CD357 on UniProt.org

Related FAQs

I see dim staining with anti human CD357 (GITR) clone 621 in blood and PBMCs.
Our testing has found that anti human CD357 (GITR) clone 621 stains dim with blood and PBMCs and may only be suitable for situations where GITR levels are high, i.e. stimulated cells or specific cell lines expressing high levels of GITR.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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