APC anti-human CD195 (CCR5) Phospho (Ser349) Antibody

Pricing & Availability
Clone
E11/19 (See other available formats)
Other Names
CD195, CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
E11slash19_APC_1_060311
PMA (100 ng/ml)-stimulated (5 minutes) human peripheral blood lymphocytes surface stained with CD3 FITC and intracellularly stained with CD195 (clone E11/19) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • E11slash19_APC_1_060311
    PMA (100 ng/ml)-stimulated (5 minutes) human peripheral blood lymphocytes surface stained with CD3 FITC and intracellularly stained with CD195 (clone E11/19) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • E11slash19_APC_2_060311
See APC spectral data
Cat # Size Price Quantity Avail. Save
321608 100 tests $275
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Description

CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Phosphopeptide (CEAPERA(pS)(pS)VYTR(pS)TGEQI(pS)VGL) of CCR5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The E11/19 monoclonal antibody binds to phosphoserine 349 corresponding to 22 c-terminal amino acid residues of human CCR5 and is useful for flow cytometric analysis, immunoblotting, and ELISA assay of CCR5 expression. Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1, and ELISA detection1 when paired with T21/8 as the capture antibody (Cat. No. 321401/321402).

Application References

(PubMed link indicates BioLegend citation)
  1. Pollok-Kopp B, et al. 2003. J. Biol. Chem. 278:2190. (IF ELISA)
  2. Fox JM, et al. 2011. 117:1851. PubMed
RRID
AB_10900994 (BioLegend Cat. No. 321608)

Antigen Details

Structure
G-coupled receptor family 1, membrane protein, 45 kD
Distribution
Subset of T cells, monocytes
Function
Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1.
Ligand Receptor
MIP-1a, MIP-1ß, RANTES
Cell Type
T cells, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, Phospho-Proteins
Antigen References

1. Samson M, et al. 1996. Biochemistry 35:3362.
2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161.
3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147.
4. Deng H, et al. 1996. Nature 381:661.

Gene ID
1234 View all products for this Gene ID
UniProt
View information about CD195 pS355 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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