APC anti-human CD114 (G-CSFR) Antibody

Pricing & Availability
Clone
LMM741 (See other available formats)
Workshop
VI MA98
Other Names
CSF3R, HG-CSFR, Colony Stimulating Factor 3 receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
LMM741_APC_021810
Human peripheral blood granulocytes from stained with LMM741 APC
  • LMM741_APC_021810
    Human peripheral blood granulocytes from stained with LMM741 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
346107 25 tests $125
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346108 100 tests $280
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Description

CD114 is the receptor of the colony stimulating factor 3 (CSF3, G-CSF), consist of two 130 kD, type I transmembrane chains that form a homodimer. The extracellular domain consists of an immunoglobulin-like domain, a cytokine receptor homologue domain, and three fibronectin type III repeats. CD114 is expressed in all stages of granulocyte differentiation and in monocytes, platelets, endothelial cells, placenta and trophoblasts. The binding of CSF3, results in the activation of many signaling molecules such as Syk, Lyn, Jak1, Jak2, Tyk2, SOCS3, SOCS1, STAT5, and Shp1, resulting in the expression of different target genes that will increase neutrophil precursor survival, proliferation and maturation. In mature neutrophils, it increases survival, superoxide anion generation, arachidonic acid release, production of myeloperoxidase and leukocyte alkaline phosphatase (LAP).

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CHO cells transfected with hG-CSFR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Layton JE, et al. 1997. Growth Factors. 14:117
  2. Layton JE, et al. 1999. J. Biol. Chem. 274:17445
  3. Layton JE, et al. 2001. J. Biol. Chem. 276:36779
  4. Gupta K, et al. 2014. Blood. 123:2550. PubMed
Product Citations
  1. Gupta K, et al. 2014. Blood. 123:2550. PubMed
  2. Bräuer-Hartmann D, et al. 2015. Cancer Res . 75: 3411-3424. PubMed
  3. Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed
Publication Library
RRID
AB_2229794 (BioLegend Cat. No. 346107)
AB_2083866 (BioLegend Cat. No. 346108)

Antigen Details

Structure
Single chain type I transmembrane molecule of 130 kD. The extracellular domain consists of an immunoglobulin-like domain, a cytokine receptor homologue domain, and three fibronectin type III repeats. To function as a receptor, this molecules form a homod
Distribution
Granulocytes (in all stages of differentiation), monocytes, platelets, endothelial cells, placenta and trophoblasts.
Function
Stimulates survival, proliferation, and maturation of neutrophil precursors. In mature neutrophils increases survival, superoxide anion generation, arachidonic acid release, production of myeloperoxidase and leukocyte alkaline phosphatase (LAP).
Interaction
Syk, Lyn, Jak1, Jak2, Tyk2, SOCS3, SOCS1, STAT5, Shp1.
Ligand/Receptor
CSF3(G-CSF)
Bioactivity
Stimulate the proliferation and differentiation of neutrophils precursors and the activation of mature neutrophils.
Cell Type
Granulocytes, Monocytes, Platelets, Endothelial cells, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Starnes LM, et al. 2009. Blood 114:1753
2. Skokowa J, et al. 2009. Nat Med. 15:151
3. Ai J, et al. 2008. PLoS One. 3:e3422
4. Germeshausen M, et al. 2008. Curr Opin Hematol. 15:332
5. Marino VJ, Roguin LP, et al. 2008. J Cell Biochem. 103:1512
6. Irandoust MI, et al. 2007. EMBO J. 2:1782

Gene ID
1441 View all products for this Gene ID
UniProt
View information about CD114 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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