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APC anti-His Tag Antibody

Pricing & Availability
Clone
J095G46 (See other available formats)
Regulatory Status
RUO
Other Names
Hexa histidine-tag, 6xHis-tag, 6XHis, 6His
Isotype
Mouse IgG2a, κ
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Product Citations
publications
J095G46_APC_His_Tag_Antibody_081517
CL hTMEM8A transfected P3.U1 stained with anti-His Tag (clone J095G46) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
  • J095G46_APC_His_Tag_Antibody_081517
    CL hTMEM8A transfected P3.U1 stained with anti-His Tag (clone J095G46) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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362605 100 tests $338
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Description

This antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.

Clone J095G46 has been verified for immunocytochemistry (ICC).

Product Citations
  1. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  2. Pham TC, et al. 2021. J Extracell Vesicles. e12057:10. PubMed
  3. Zhou H, et al. 2021. Cell. . PubMed
  4. Xiong X, et al. 2021. Liver Int. 41:1033. PubMed
  5. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  6. Huang HC, et al. 2021. EBioMedicine. 74:103712. PubMed
  7. Fu H, et al. 2021. Front Cell Dev Biol. 9:764109. PubMed
  8. Jalloh S, et al. 2022. PLoS Pathog. 18:e1010479. PubMed
  9. Huang A, et al. 2022. Antibodies (Basel). 11:. PubMed
  10. Sokal A, et al. 2021. Immunity. S1074-7613:00396. PubMed
RRID
AB_2715818 (BioLegend Cat. No. 362605)

Antigen Details

Structure
6 repeat His amino acid
Function
Epitope tag
Biology Area
Cell Biology, Immunology
Antigen References
  1. Zhao C, et al. 2010. Anal Biochem. 399:237
  2. Salichs E, et al. 2009. PLoS Genet. 5:e1000397
  3. Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523
  4. Lindner, P, et al. 1997. Biotechniques. 22:140
  5. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  6. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Gene ID
NA
UniProt
View information about His Tag on UniProt.org

Related FAQs

There are no FAQs for this product.

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Go To Top Version: 1    Revision Date: 08/18/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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