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Antigen Reactive T cell Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Lymphocyte function-associated antigen 1, LFA-1, antigen specific, T cells, CD11a/CD18 Lymphocyte function-associated antigen 1, LFA-1, antigen specific, T cells, CD11a/CD18
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A_345905_Antigen_Reactive_T_cell_kit
Kit content image for the Antigen Reactive T cell Kit
  • A_345905_Antigen_Reactive_T_cell_kit
    Kit content image for the Antigen Reactive T cell Kit
  • B_Antigen-Reactive_T-cell-Kit_1_042922
    CD4+ human peripheral blood lymphocytes were stimulated lyophilized negative control (left) or positive control (right) for 20 minutes, then stained with anti-human LFA-1 PE followed by surface cocktail. Data was analyzed using gating strategy depicted in Figure 3.
  • C_Antigen-Reactive_T-cell-Kit_2_042922
    CD8+ human peripheral blood lymphocytes were stimulated lyophilized negative control (left) or positive control (right) for 20 minutes, then stained with anti-human LFA-1 PE followed by surface cocktail. Data was analyzed using gating strategy depicted in Figure 3.
  • D_Antigen-Reactive_T-cell-Kit_3_042722
    Gating strategy using scatter parameters and surface markers in the staining cocktail to identify CD4+ and CD8+ cells.
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345905 10 tests $411
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Description

The Antigen reactive T cell kit contains negative control (vial N) and positive control (vial P) in convenient packaging for stimulating human blood or PBMCs along with anti-human LFA-1 PE and surface stain cocktail consisting of anti-human CD45 PerCP, CD8 Pacific Blue™, CD4 FITC and CD14, CD41, CD15, CD16, CD19 APC/Cyanine7. This kit contains controls that can be paired with peptides of your choice for detecting antigen specific responses as well assessing vaccine responses. Cells or beads stained with individual fluorochrome-conjugated antibodies should be used for setting up compensation before running the samples. For data analysis, we recommend following gating strategy – Time –> Singlets –> Lymphocytes –> APC/Cyanine7 (Dump) negativeCD45+ –> CD4+CD8– and CD8+CD4–. 

The Antigen reactive T cell kit provides a convenient, easy to use, and powerful tool for immunology and vaccine research.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Formulation
Each kit will contain the following components:

Kit Components:

1 vial negative control
1 vial positive control
1 empty vial (for peptide stimulation)
1 vial surface stain cocktail (5 tests)
3 tubes of LFA-1 PE (1 test each) 1 bottle 50 mL RBC Lysis/Fixation solution
1 bottle 0.5 mL EDTA 0.08M
1 bottle 500 mL Cell Staining Buffer
Preparation
Kit consists of lyophilized components.
Storage & Handling
Store the kit at 2°C - 8°C upon receipt. Do not open the sealed pouch until ready to use.
Application

FC - Quality tested
Recommended Usage

Refer to manual for detailed usage instructions.

Application References

(PubMed link indicates BioLegend citation)
  1. Dimitrov S, et al. 2018. Pro Natl Acad Sci  U S A. 115:E5536-E5545.
  2. Gouttefageas C, et al. 2019. Cancer Immunol Immunother. 68:1855-1863.
  3. Schollhorn A, et al. 2021. Front Immunol. 12:626308.
  4. Schollhorn A, et al. 2023. Front Immunol. 13:1107366.

Antigen Details

Interaction
CD4+ T cells, CD8+ T cells, antigen specificity
Ligand/Receptor
β2-integrin
Antigen References
  1. Altvater B, et al. 2012. Cancer Immunol Immunother. 61:385.
  2. Kollgaard T, et al. 2015. 10:e0131934.
  3. Grant EJ, et al. 2018. Nat Commun. 9:5427.
  4. Gary R, et al. 2018. J Transl Med. 16:124.
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/27/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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