Anti-Tubulin β-3, Polymerized (TUBB3) Antibody (Previously Covance catalog# SMI-62R)

Pricing & Availability
Clone
SMI 62 (See other available formats)
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin
Previously
Covance Catalog# SMI-62R
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
1-SMI-62_polymerized-b-tubulin_purified_IF_022415.jpg
Staining of Polymerized Beta Tubulin (SMI 62) on Hela cells using MeOH fixation.
  • 1-SMI-62_polymerized-b-tubulin_purified_IF_022415.jpg
    Staining of Polymerized Beta Tubulin (SMI 62) on Hela cells using MeOH fixation.
  • 2-SMI-62_Tubulin_beta-3_Polymerized_Antibody_WB_120417
    Total lysates (15 µg protein) from HepG2 and HeLa cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 anti-Tubulin β-3, Polymerized (TUBB3) antibody, clone SMI 62. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 3-SMI-62_Tubulin_beta-3_Polymerized_Antibody_WB_060118
    Whole cell lysates (15 µg protein) from A549 (lane 1) and HeLa (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Tubulin Beta 3 (TUBB3) antibody, clone AA10, or anti-Tubulin β-3, Polymerized (TUBB3) antibody (clone SMI 62) (1:1000 dilution). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-Actin antibody (1:5000 dilution, Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder. Cell lysates were loaded in order of decreasing TUBB3 mRNA expression levels; A549 cells express ~14-fold more TUBB3 mRNA than HeLa cells (source: Human Protein Atlas). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
921001 500 µl $296
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Description

Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration, and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of α- and β-tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. TUBB3 is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance.

Product Details
Technical data sheet

Product Details

Reactivity
Mammalian, Green Algae, Molds
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Ascites Fluid (contains 0.01M sodium azide).
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

ICC - Quality tested
ELISA, WB

Recommended Usage

The optimal working dilution should be determined for each specific assay condition. The extent of permissible dilution of SMI 62 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.
WB: 1:1,000
ICC: 1:1,000
ELISA: 1:1,000

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC), and ELISA.

SMI 62 reacts with polymerized β-tubulin. The antibody has a preference for recognizing polymerized tubulin in immunocytochemistry. This antibody helps reduce background in cell staining applications caused by unpolymerized tubulin in the cytoplasm. Reaction is observed with cold-stabilized tubulin, premitotic, interphase and mitotic nuclei and flagellar tubulin. SMI 62 reacts with most mammalian species in neuronal and non-neuronal tissue. Green algae and molds also react with SMI 62.
 

Observed MW is at 50 kDa.

This clone shows high reactivity towards other β–tubulin isotypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhu B, et al. 2010. Am J Physiol Lung Cell Mol Physiol. 299: L493. (WB) PubMed
  2. Fortune B, et al. 2008. Invest. Ophthalmol. Vis. Sci. 49: 255.
Product Citations
  1. Zhu B, et al. 2010. Am J Physiol Lung Cell Mol Physiol. 299:L493-501. PubMed
Publication Library
RRID
AB_2565385 (BioLegend Cat. No. 921001)

Antigen Details

Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Gene ID
203068 View all products for this Gene ID
UniProt
View information about Tubulin beta 3, Polymerized (TUBB3) on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 12/04/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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