Anti-Synuclein-α/β, 130-140 Antibody (Previously Covance catalog# MMS-529R)

Pricing & Availability
Clone
Syn 202 (See other available formats)
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease (autosomal dominant, Lewy body) 4
Previously
Covance Catalog# MMS-529R
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
A_Syn202_Ascites_SynucleinAB_IHCP_052118
IHC staining of anti-Synuclein-α/β, 130-140 antibody (clone Syn 202) on formalin-fixed paraffin-embedded human Parkinson's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with the primary antibody at 1:500 dilution overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_Syn202_Ascites_SynucleinAB_IHCP_052118
    IHC staining of anti-Synuclein-α/β, 130-140 antibody (clone Syn 202) on formalin-fixed paraffin-embedded human Parkinson's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with the primary antibody at 1:500 dilution overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_Syn202_Ascites_SynucleinAB_WB_052118
    Western blot of anti-Synuclein-α/β, 130-140 antibody (clone Syn 202). Lane 1: Molecular weight marker; Lane 2: 20 µg of human Parkinson's disease brain lysate; Lane 3: 20 µg of normal human cerebral cortex lysate; Lane 4: 20 µg of mouse brain lysate. The blot was incubated with the primary antibody diluted at 1:500 dilution overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
836602 25 µl $125
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836601 200 µl $320
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Description

Synucleins are small soluble proteins expressed primarily in neural tissues and in certain tumors. The family includes 3 known proteins, alpha synuclein, beta synuclein and gamma synuclein. All synucleins have in common a highly conserved alpha helical lipid binding motif with similarity to the class-A2 lipid binding domains of the exchangeable apolipoproteins. The alpha and beta synuclein proteins are found primarily in brain tissue, where they are seen mainly in pre-synaptic terminals. Alpha synuclein is believed to be a major component of Lewy bodies in Parkinson's disease. Mutations in alpha synuclein are associated with rare familial cases of early onset Parkinson's disease, Alzheimer's disease and several other neurodegenerative illnesses.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Syn 202 was raised using recombinant alpha and beta synucleins and recognizes residues 130-140 in alpha and beta synuclein.
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

IHC-P - Quality tested
WB - Validated
EM, ICC, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:500-1:1000 is suggested. For Western blotting, the suggested dilution of this reagent is 1:500-1:1000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunoprecipitation (IP), and immunohistochemistry of formalin-fixed paraffin-embedded tissue sections (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. Nibe K, et al. 2009. Vet. Pathol. 46(3):474-83. (IHC)
  2. Giasson BI, et al. 2000. J. Neurosci. Res. 59(4):528-33.
  3. Mazzulli JR, et. al. 2011. Cell 146:37. (WB)
  4. Giasson BI, et. al. 1999. J. Biol. Chem. 274:7619. (EM)
  5. Giasson BI, et. al. 2002. Neuron. 34:521. (ICC)
  6. Usenovic M, et. al. 2012. J. Neurosci. 32:4240. (WB) PubMed
  7. Morrison GJ, et. al. 2012. PLoS One 7:e43288. (IP)
  8. Knight AL, et. al. 2014. Cell Metab. 20:145. (WB) PubMed
Product Citations
  1. Usenovic M, et al. 2012. J Neurosci. 32:4240-4246. PubMed
  2. Knight A, et al. 2014. Cell Metab. 20:145-157. PubMed
Publication Library
RRID
AB_2734606 (BioLegend Cat. No. 836602)
AB_2565365 (BioLegend Cat. No. 836601)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Gene ID
6622 View all products for this Gene ID
UniProt
View information about Synuclein-alpha/beta, 130-140 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/21/2018

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