Anti-Synaptophysin Antibody (Previously Covance catalog# MMS-618R)

Pricing & Availability
Clone
SP17 (See other available formats)
Other Names
SYP, Synaptophysin, Major synaptic vesicle protein P38
Previously
Covance Catalog# MMS-618R
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
SP17_Ascites_Synaptophysin_Antibody_1_101518
Western blot of anti-Synaptophysin antibody (clone SP17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.
  • SP17_Ascites_Synaptophysin_Antibody_1_101518
    Western blot of anti-Synaptophysin antibody (clone SP17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.
  • SP17_Ascites_Synaptophysin_Antibody_2_101518
    IHC staining of anti-Synaptophysin antibody (clone SP17) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP17_Ascites_Synaptophysin_Antibody_3_101518
    IHC staining of anti-Synaptophysin antibody (clone SP17) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP17_Ascites_Synaptophysin_Antibody_4_101518
    ICC staining of anti-Synaptophysin antibody (clone SP17) on U251MG cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with a 1:5000 dilution of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLongTM Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
837102 25 µl $90
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837101 500 µl $369
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Description

Synaptophysin, also known as major synaptic vesicle protein P38, is encoded by the SYP gene in humans and is located on the short arm of the X chromosome. Synaptophysin is located in neuroendocrine cells as well as all neurons in the CNS that participate in synaptic transmission. It is a 38 kD protein that contains four transmembrane domains. Synaptophysin is a synaptic vesicle glycoprotein, and interacts with synaptobrevin. It has been implicated in X-linked mental retardation and can be used as a specific marker for cells of the adrenal medulla and pancreatic islets. As such, it can be used to identify tumors that are derived from those cells, including neuroblastoma, retinoblastoma, medulloblastoma, and others.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a crude synaptic preparation from post mortem human brain.
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB - Quality tested
IHC-P, ICC - Validated
ELISA - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 - 1:5000 dilution. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a dilution range of 1:1000 - 1:5000 is suggested. For immunocytochemistry, a dilution range of 1:1000 - 1:5000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The antibody is effective in immunoblotting (WB), immunohistochemistry (IHC-P), immunocytochemistry (ICC), and ELISA.

SP17 reacts with native, denatured and recombinant synaptic vesicle protein synaptophysin expressed in CHO cells. The antibody is particularly valueable for immunocytochemical studies of the brain due to its stability in formalin sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Honer WG, et al. 1993. Brain Res. 609:9-20.
  2. Honer WG, et al. 1997. Neuroscience. 78:99-110.
  3. Frigo DE and McDonnell DP. 2008. Mol. Cancer Ther. 3:659. (WB) PubMed
  4. Wolf AB, et al. 2012. J Alzheimers Dis. 1:217. (ELISA) PubMed
Product Citations
  1. Wolf A, et al. 2012. J Alzheimers Dis. 32:217-232. PubMed
  2. McDonnell D 2008. Mol Cancer Ther. 7:659-669. PubMed
Publication Library
RRID
AB_2734609 (BioLegend Cat. No. 837102)
AB_2565370 (BioLegend Cat. No. 837101)

Antigen Details

Structure
Predicted MW = 40 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
6855 View all products for this Gene ID
UniProt
View information about Synaptophysin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10/15/2018

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