Anti-RNA Polymerase TFIIB ImmunoAffinity Resin

Pricing & Availability
Clone
IIB8 (See other available formats)
Other Names
Softag 3
Previously
NeoClone Catalog# S0003
Isotype
Mouse IgG2a
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Cat # Size Price Quantity Avail. Save
673701 1 mL $275
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Description

Softag purification takes advantage of the unique properties of a class of monoclonal antibodies, termed polyol-responsive monoclonal antibodies which can be used in immunoaffinity purification. The advantage of the Polyol-Responsive mAb System is that the target protein is not denatured during purification, so it is suitable for the purification of labile, multi-subunit protein complexes. These protein complexes bind tightly to the polyol-responsive mAbs, but they are released under very gentle elution conditions. Additionally, the proteins are highly pure compared to other systems.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
50% anti-RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Storage & Handling
Store at 4°C until use. Use at room temperature.
Application

IP - Quality tested

Recommended Usage

Resin binding capacity is greater than 0.3 mg/mL (based on 28 kD tagged protein). To elute, use TE, 0.7M NaCl, 30% propylene glycol.
 

Application Notes

Previously item# S0003 from Neoclone.

This resin is also appropriate for use in identifying proteins associated with the recombinant protein tagged with Softag 3. Softag 3 is an immunoaffinity system appropriate for purifying tagged proteins from prokaryotic expression sources. The epitope (TQDPSRVG) is derived from a subunit of eukaryotic transcription factor TFIIB.

Application References

(PubMed link indicates BioLegend citation)
  1. Duellman SJ, et al. 2004. Protein Expr. Purif. 35:147.

Antigen Details

Structure
TFIIB is a 35 kD human transcription factor
Biology Area
Cell Biology
Molecular Family
Nuclear Markers
Gene ID
2959 View all products for this Gene ID
UniProt
View information about RNA Polymerase TFIIB on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 03/11/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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