Anti-Neurofilament M (NF-M) Antibody (Previously Covance catalog# PRB-575C)

Pricing & Availability
Clone
Poly28410 (See other available formats)
Other Names
160 kD Neurofilament Protein, Neurofilament Triplet M Protein, Neurofilament-3 (150 Kd Medium), Neurofilament, Medium Polypeptide 150kD
Previously
Covance Catalog# PRB-575C
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
841001 100 µl $266
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Description

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, a-internexin, nestin and in some cases vimentin. Antibodies in the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse, Cat, Chicken
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The immunogen was the extreme carboxyterminal region of rat NF-M, which was expressed in E. coli and purified by ion exchange chromatograpy.
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

IHC, WB, IF

Recommended Usage

Each lot of this antibody is quality control tested by immnohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:500
WB: 1:5,000-10,000*
IF: 1:500-1,000

Tissue Sections: Formalin-fixed, paraffin-embedded tissue.
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval Solution (Cat. No. 928501, 928601).
Incubation: 20 minutes, room temperature.

Positive Control: Rat brain tissue

Application Notes

This antiserum is effective in immunoblotting (WB) and immunofluorescence (IF).

* Predicted MW = 145-160 kD

The production and characterization of this antiserum similar to monoclonal NF-M clone 3H11.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Harris J, Ayyub C, Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J. Neurosci. Res. 30:47-62, 1991.
RRID
AB_2565457 (BioLegend Cat. No. 841001)

Antigen Details

Cell Type
Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
4741 View all products for this Gene ID
UniProt
View information about Neurofilament M (NF-M) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/14/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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