Anti-Neurofilament M (NF-M) Antibody (Previously Covance catalog# PRB-575C)

Pricing & Availability
Poly28410 (See other available formats)
Other Names
160 kD Neurofilament Protein, Neurofilament Triplet M Protein, Neurofilament-3 (150 Kd Medium), Neurofilament, Medium Polypeptide 150kD
Covance Catalog# PRB-575C
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
Cat # Size Price Quantity Avail. Save
841001 100 µl $266
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, a-internexin, nestin and in some cases vimentin. Antibodies in the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable.

Product Details
Technical data sheet

Product Details

Human, Rat, Mouse, Cat, Chicken
Antibody Type
Host Species
The immunogen was the extreme carboxyterminal region of rat NF-M, which was expressed in E. coli and purified by ion exchange chromatograpy.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.


Recommended Usage

Each lot of this antibody is quality control tested by immnohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:500
WB: 1:5,000-10,000*
IF: 1:500-1,000

Tissue Sections: Formalin-fixed, paraffin-embedded tissue.
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval Solution (Cat. No. 928501, 928601).
Incubation: 20 minutes, room temperature.

Positive Control: Rat brain tissue

Application Notes

This antiserum is effective in immunoblotting (WB) and immunofluorescence (IF).

* Predicted MW = 145-160 kD

The production and characterization of this antiserum similar to monoclonal NF-M clone 3H11.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Harris J, Ayyub C, Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J. Neurosci. Res. 30:47-62, 1991.
AB_2565457 (BioLegend Cat. No. 841001)

Antigen Details

Cell Type
Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
4741 View all products for this Gene ID
View information about Neurofilament M (NF-M) on

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/14/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Forgot your password? Reset Password
Request an Account