Anti-Nestin Antibody (Previously Covance catalog# PRB-570C)

Pricing & Availability
Poly18419 (See other available formats)
Other Names
Covance Catalog# PRB-570C
Rabbit Polyclonal IgG
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Product Citations
Rabbit anti-human nestin staining of a human glioblastoma cell line, U251. Nestin is stained in green, blue is bisbenzimide. Photo courtesy of J. Hou, NIH.
  • Poly18419_Nestin_Serum_IF_030315.jpg
    Rabbit anti-human nestin staining of a human glioblastoma cell line, U251. Nestin is stained in green, blue is bisbenzimide. Photo courtesy of J. Hou, NIH.
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841901 100 µL $325
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Nestin, a class IV intermediate filament protein is expressed during early stages of development in the CNS and in myogenic tissues. Upon differentiation, nestin becomes down regulated and is replaced by other tissue specific intermediate filaments. Antibodies against nestin can be used as a predominant marker to describe stem and neural progenitor cells in the mammalian CNS.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
The polyclonal nestin antibody was raised using a GST fusion protein.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:5,000*
IHC: 1:200
IF: 1:200

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC) and immunohistochemistry (IHC) applications.

*Predicted MW = 220-240 kD

Using this antiserum, nestin expression was detected in progenitor cells and in a small subset of neurons. In addition, nestin was also detected in cells that were in transition from a progenitor stage to glial differentiation.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Messam CA, et al. 2000. Exp Neurol. 161:585-96.
  2. Ueno M, et al. 2006. PNAS. 103:9554.
  3. Nagase T, et al. 2009. Dev Dyn. 238:1118.
  4. Watanabe K, et al. 2007. Nat Biotechnol. 25:681.
  5. Sosunov AA, et al. 2013. J Neurosci. 33:7439-50. [IF] PubMed
Product Citations
  1. MOSES C, et al. 2020. Sci Rep. 10:11393. PubMed
  2. Sosunov A, et al. 2013. J Neurosci. 33:7439-7450. PubMed
  3. Momcilovic O, et al. 2016. PLoS One. 11: 0154890. PubMed
AB_2565468 (BioLegend Cat. No. 841901)

Antigen Details

Cell Type
Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Intermediate Filaments
Gene ID
10763 View all products for this Gene ID
View information about Nestin on

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Go To Top Version: 3    Revision Date: 01/07/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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