Anti-mCherry Nanobody Affinity Gel

Pricing & Availability
Clone
LaM-4 (See other available formats)
Other Names
DSRED
Isotype
Derived from IgGVHH.
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Product Citations
publications
LaM-4_Affinity Gel_mCherry_08312016
mCherry-fused protein was immunoprecipitated from 100 µg transfected 293E cell extract using 10 µl anti-GFP nanobody Affinity Gel (lane 1, negative control) and 10 µl anti-mCherry nanobody Affinity Gel (lane 2). Immunoprecipitates and 10% of total input (10 µg, Lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with Direct-Blot™ HRP anti-mCherry Antibody (clone 8C5.5). Proteins were visualized using chemiluminescence detection.
  • LaM-4_Affinity Gel_mCherry_08312016
    mCherry-fused protein was immunoprecipitated from 100 µg transfected 293E cell extract using 10 µl anti-GFP nanobody Affinity Gel (lane 1, negative control) and 10 µl anti-mCherry nanobody Affinity Gel (lane 2). Immunoprecipitates and 10% of total input (10 µg, Lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with Direct-Blot™ HRP anti-mCherry Antibody (clone 8C5.5). Proteins were visualized using chemiluminescence detection.
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689502 1 ml $425
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Description

mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins. mCherry derives from proteins initially isolated from Cnidarians.


Nanobodies are heavy chain only, single-domain antibodies, that lack the presence of light chains. These are typically derived from members of the Camelidae family that include llamas and camels. Their variable region (VHH) is the smallest antigen-binding fragment found in a natural antibody, and nanobodies are the smallest (≈15 kD) naturally occuring immunoglobins. Further, nanobodies are stable, and can bind antigens with high affinity. Experimentally, nanobodies have been applied in WB, IF, and IP. In IP/WB applications, nanobodies can avoid IgG heavy/light chain issues that occur when using regular antibodies.

Product Details
Technical data sheet

Product Details

Reactivity
Discosoma mCherry
Antibody Type
Recombinant
Immunogen
Recombinant mCherry.
Formulation
50% anti-mCherry nanobody (clone LaM-4) conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Upon receipt, store between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Application

IP - Quality tested

Recommended Usage

For immunoprecipitation, the suggested use of this reagent is 5 - 20 µl affinity gel for 100 µg lysate harvested from mCherry overexpressed cells.

Antigen Details

Structure
mCherry is 236 amino acids with a predicted molecular weight of approximately 27 kD.
Function
Fluorescent protein.
Biology Area
Cell Biology
Antigen References

1. Shaner NC, et al. 2008. Nat. Methods. 6:545-51.
2. Shaner NC, et al. 2005. Nat. Methods 12:905-9.
3. Cortez-Retamozo V, et al. 2004. Cancer Res. 64:2853.
4. Muyldermans S. 2013. Annu. Rev. Biochem. 82:775.
5. Fridy PC, et al. 2014. Nat. Methods 11:1253.

Gene ID
NA
UniProt
View information about mCherry on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 04/05/2018

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