Anti-human CD3 PE-Cy5/CD4 PE/CD8 FITC Cocktail

Pricing & Availability
Clone
UCHT1/RPA-T4/RPA-T8 (See other available formats)
Isotype
Mouse IgG1 (all clones)
Ave. Rating
Submit a Review
Product Citations
publications
Liquid_Antibody_Cocktail_1_022113
  • Liquid_Antibody_Cocktail_1_022113
  • Liquid_Antibody_Cocktail_2_022113
  • Liquid_Antibody_Cocktail_3_022113Liquid_Antibody_Cocktail_3_022113
    Human peripheral blood lymphocytes were stained with anti-human CD3 PECy5/CD4 PE/CD8 FITC cocktail.
Cat # Size Price Quantity Avail. Save
319001 50 tests $275
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes.
CD4: RPA-T4 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages.
CD8: RPA-T8 antibody reacts with CD8a, a 32-34 kD type I glycoprotein. CD8a forms a heterodimer or homodimer with CD8b. CD8 also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (express almost exclusively CD8a homodimers).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent is a combination of PE/Cy5 conjugated UCHT1, PE conjugated RPA-T4 and FITC conjugated RPA-T8 at optimal concentration for three-color flow cytometric analysis.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
This product is guaranteed for nine months. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.

This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.
Application

FC - Quality tested

Recommended Usage

For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood.

Application Notes

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

Note:
Single color controls are required to perform instrument compensation and are not included in the kit.  

RRID
AB_316003 (BioLegend Cat. No. 319001)

Antigen Details

Distribution

T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes

Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
4. Center D, et al. 1996. Immunol. Today 17:476.
5. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID 915 View all products for this Gene ID 920 View all products for this Gene ID 925 View all products for this Gene ID
UniProt
View information about CD3/CD4/CD8 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12/03/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account