Anti-human CD3 FITC/(CD16+CD56) PE Cocktail

Pricing & Availability
Clone
UCHT1/3G8+MEM-188 (See other available formats)
Isotype
Mouse IgG1/IgG1/IgG2a
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
319101 50 tests $195
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Description

CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes.
CD16: 3G8 antibody reacts with CD16, a 50-65 kD transmembrane or a 48 kD GPI-anchored glycoprotein that is a member of the immunoglobulin superfamily. CD16 is expressed on NK cells, activated monocytes, macrophages, mast cells and neutrophils.
CD56: MEM-188 antibody reacts with the 175 - 185 kD isoform of CD56, N-CAM (Neural Cell Adhesion Molecule), which is expressed on NK cells and a subset of CD3+ T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent is a combination of FITC conjugated UCHT1 and PE conjugated 3G8 and MEM-188 in optimal concentration for two-color flow cytometric analysis.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Note:
Single color controls are required to perform instrument compensation and are not included in the kit. 

Product Citations
  1. Jeff Baker, Joshua Nederveen, Gianni Parise 2017. J Appl Physiol. 122(1):182-190. PubMed
Publication Library
RRID
AB_314999 (BioLegend Cat. No. 319101)

Antigen Details

Distribution

T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes

Cell Type
Mesenchymal Stem Cells, NKT cells, T cells, Thymocytes
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
4. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
5. Stroncek D, et al. 1991. Blood 77:1572.
6. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.
7. Lanier L, et al. 1991. J. Immunol. 146:4421.
8. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
9. Cremer H, et al. 1994. Nature 367:455.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD3/CD16+CD56 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 12/03/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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