- SMI 26 (See other available formats)
- Other Names
- Glial fibrillary acidic protein
Covance Catalog# SMI-26R
- Mouse IgG2b and IgG1 (SMI 21)
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- Product Citations
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GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.Product Details
- Human, Sheep, Cow, Dog, Pig, Rat, Guinea pig, Rat, Mouse, Chicken
- Antibody Type
- Host Species
- Ascites Fluid (contains 0.01M sodium azide).
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
IHC, WB, ICC, ELISA
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The extent of permissible dilution of SMI 26 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.
• WB: 1:1,000
• ICC: 2:250-500
• IHC: 1:250-500
• ELISA: 1:1,000
Tissue Sections: Formalin-fixed, paraffin-embedded tissues & frozen sections
Pretreatment: Not required
Incubation: 60 minutes at room temperature
- Application Notes
This antibody cocktail is effective in immunoblotting, immunocytochemistry, immunohistochemistry and ELISA.
The Bigner-Eng antibodies have originally been assayed by indirect radioimmunoassay against fixed cell monolayers of a GFAP-positive human glioma cell line and also by competitive radioimmunoassay with radiolabelled GFAP and by competitive immunoradioassay with radiolabelled antibody. They have been further characterized by immunoblots of GFAP and by immunocytochemistry. Each of the Bigner-Eng antibodies is specific for GFAP and visualizes immunocytochemically astrocytes and astrocytic processes as well as Bergman glia in a wide variety of species. Mixtures of the three Bigner-Eng antibodies provide for a more comprehensive detection of astrocytomas than each antibody alone. Both, anaplastic and reactive astrocytes are stained immunocytochemically. Metastatic tumors and brain tumors of non-astrocytic origin, such as medulloblastomas, meningiomas, choroid plexus papillomas and schwannomas are not stained. There appears to be a positive correlation between fibrillarity of immunocytochemical localization in astrocytomas and their degrees of differentiation, and a negative correlation between the percentage of positive cells and their degree of anaplasticity. In morphologically diagnosed ependymomas and oligodendrogliomas positive reaction appears to reveal presence of malignant astrocytes. SMI 26 is a cocktail of GFAP antibodies SMI 21 (mouse monoclonal IgG1) and SMI 23, SMI 24, and SMI 25 (all mouse monoclonal IgG2b) for a comprehensive visualization of astrocytomas.
(PubMed link indicates BioLegend citation)
- Chen MH, Hagemann TL, Quinlan RA, Messing A, Perng MD. Caspase Cleavage of GFAP Produces an Assembly-Compromised Proteolytic Fragment that Promotes Filament Aggregation. ASN Neuro; 5: AN20130032, Oct 2013. (IF, WB) PubMed
- Product Citations
AB_2565379 (BioLegend Cat. No. 837601)
AB_2565380 (BioLegend Cat. No. 837602)
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