Anti-GFAP Antibody (Previously Covance catalog# PRB-571C)

Pricing & Availability
Clone
Poly28400 (See other available formats)
Other Names
Glial Fibrillary Acidic Protein
Previously
Covance Catalog# PRB-571C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Purified_GFAP_Antibody_IHC_1_030415
Rat cortical neurons and glia in mixed tissue culture stained with NF-H (red) and GFAP (green). The nuclei are stained with Hoescht dye (blue). Photo courtesy of EnCor Biotechnology Inc.
  • Purified_GFAP_Antibody_IHC_1_030415
    Rat cortical neurons and glia in mixed tissue culture stained with NF-H (red) and GFAP (green). The nuclei are stained with Hoescht dye (blue). Photo courtesy of EnCor Biotechnology Inc.
  • Purified_GFAP_Antibody_IHC_2_030415
    Rat hippocampus stained with rabbit anti-GFAP, (red, AF568) and chicken anti-NF-H, (green, AF488). Photo courtesy of Molecular Expressions.
Cat # Size Price Quantity Avail. Save
840001 100 µl $266
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Description

Glial Fibrillary Acidic Protein (GFAP) was found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. The GFAP protein runs on gels at ~55kD protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene. All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, Cat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antiserum was made with a preparation of recombinant GFAP expressed in bacteria and highly purified. Subsequent boosts were performed with purified GFAP from bovine spinal cord.
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB, IF, ICC

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:10,000*
IF: 1:1,000 (when using fluorescent secondary ab) or 1:5,000 (when using peroxidase labeled secondary ab)

Application Notes

This antiserum is effective in immunoblotting (WB), immunofluorescence (IF) and immunocytochemistry (ICC).

* Predicted MW = 55 kD & may see one at 48 kD (a breakdown product)


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Brenner M et al. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat. Genet. 27:117-120, 2001.
  2. Liem RKH et al. Intermediate filaments in nervous tissues. J. Cell Biol. 79:637-745, 1979.
  3. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 1972.
Product Citations
  1. Miyamoto Y, et al. 2016. Nat Commun. 7:13478. PubMed
Publication Library
RRID
AB_2565444 (BioLegend Cat. No. 840001)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/09/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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