Anti-DYKDDDDK Tag (L5) Affinity Gel

Pricing & Availability
Clone
L5 (See other available formats)
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
Ave. Rating
2 reviews
Product Citations
publications
L5_gel_graph_113011
Anti-DYKDDDDK tag affinity resin (L5-agarose) retains binding capacity even after 10 rounds of purification and elution.
  • L5_gel_graph_113011
    Anti-DYKDDDDK tag affinity resin (L5-agarose) retains binding capacity even after 10 rounds of purification and elution.
  • pos2_L5_gel_113011
    Anti-DYKDDDDK tag affinity resin (L5-agarose) retains binding capacity even after 10 rounds of purification and elution.
Cat # Size Price Quantity Avail. Save
651501 500 µl $175
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651502 2 ml $385
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651503 5 ml $695
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical data sheet

Product Details

Reactivity
DYKDDDDK tag epitope
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
50% anti-DYKDDDDK-tag (L5) antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Upon receipt, store between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Application

IP, Purification of DYKDDDDK-tagged fusion proteins from cell lysates - Quality tested

Recommended Usage

Resin binding capacity is greater than 0.8 mg/mL.

Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. Grimley R, et al. 2012. PLoS One. 7:e39847. PubMed
  2. Vervliet T, et al. 2015. Biochem Biophys Res Commun. 460:291. PubMed
  3. Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed
Publication Library

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

Does a relatively low pH such as 4.5 dissociate the L5 antibody from the resin?
pH4.5 should not diassociate the antibody from the resin. But this pH could affect the binding of the FLAG-tagged target to the L5 antibody.
Go To Top Version: 2    Revision Date: 03/14/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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