Anti-Alpha Smooth Muscle Actin Antibody (Previously Covance catalog# MMS-466S)

Pricing & Availability
Clone
1A4 (See other available formats)
Workshop
HCDM listed
Other Names
Actin, aortic smooth muscle, alpha 2 actin, alpha-actin-2, alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein
Previously
Covance Catalog# MMS-466S
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
1A4_Smooth-Muscle-Actin_Antibody_IHC_041515
Immunohistochemical staining of Smooth Muscle Actin (1A4) Monoclonal Antibody, on formalin fixed paraffin embedded human breast tissue at 1/50 dilution.
  • 1A4_Smooth-Muscle-Actin_Antibody_IHC_041515
    Immunohistochemical staining of Smooth Muscle Actin (1A4) Monoclonal Antibody, on formalin fixed paraffin embedded human breast tissue at 1/50 dilution.
Cat # Size Price Quantity Avail. Save
904601 250 µl $300
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Description

Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins being expressed in mammals and birds. The actin protein consists of six isoforms, varying in their amino acid sequence, but all having the same molecular mass of 42 kD. The isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. The N-terminal region appears to be a major antigenic region. There are different α isoforms specific for muscle tissues, i.e. skeletal muscle α cardiac muscle α and smooth muscle α respectively. The β- and γ-actins may be present in muscle cells as well as most other cell types in the body, including non-muscle cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, Cow, Chicken
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against the N-terminal synthetic decapeptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).
Formulation
Cell Culture Supernatant (dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3).
Preparation
Tissue Culture Supernatant
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store between 2°C and 8°C.
Application

IHC, ICC, WB

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:100*
IHC: 1:50-1:100**

Paraffin sections: This antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heat-induced epitope retrieval is recommended. Optimal results are obtained with 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0. Pre-treatment of tissues with proteinase-K was found destructive of the epitope. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure.
Frozen sections and cell preparations: The antibody can also be used for labelling acetone-fixed, frozen sections.

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC) and immunohistochemistry (IHC).

*Predicted MW = 42 kD

**Reccomend 20 minute heat-induced epitope retrieval in 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0, and 30 minute incubation of primary antibody at room temperature.

Application References

(PubMed link indicates BioLegend citation)
  1. Brennan PA, et al. 2000. J Oral Pathol Med. 29:279.
  2. Rizeq MN, et al. 1994. Hum Pathol. 25:671.
  3. Schmitt-Graff A, et al. 1991. Am J Pathol. 138:1233.
  4. Roholl PJM, et al 1990. Hum Pathol. 21:1269.
  5. Alberts B, et al. 1989. Eds. Molecular biology of the cell. 2:613.
  6. Skalli O, et al. 1986. J Cell Biol. 103:2787.
Product Citations
  1. Nam MO, et al. 2018. Oncotarget. 9:6356. PubMed
  2. Chozinski TJ, et al. 2018. Sci Rep. 15:4005. PubMed
Publication Library
RRID
AB_2565041 (BioLegend Cat. No. 904601)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Gene ID
59 View all products for this Gene ID
UniProt
View information about Alpha Smooth Muscle Actin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07/20/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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