- ME-9F1 (See other available formats)
- Other Names
- S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
- Rat IgG2a
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- Product Citations
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CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.Product Details
- Antibody Type
- Host Species
- Endothelial cell line TME-3H3
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-F - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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(PubMed link indicates BioLegend citation)
- Schrage A, et al. 2008. Histochem. Cell. Biol. 129:441.
- Birbrair A, et al. 2013. Am J Physiol Cell Physiol. 305:1098. PubMed
AB_2728156 (BioLegend Cat. No. 134719)
AB_2728157 (BioLegend Cat. No. 134720)
- CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.
- CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
- Unidentified ligand.
- Cell Type
- Endothelial cells, Neutrophils, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Despoix N, et al. 2008. Eur. J. Immunol. 38:2855.
2. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
3. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746.
- Gene ID
- 84004 View all products for this Gene ID
- View information about CD146 on UniProt.org
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