Alexa Fluor® 700 anti-mouse Lineage Cocktail with Isotype Ctrl

Pricing & Availability
Clone
17A2/RB6-8C5/RA3-6B2/Ter-119/M1/70 (See other available formats)
Other Names
Lin-1
Isotype
Rat IgG2b/Rat IgG2a
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Lineage_Cocktail_AF700_Antibody_FC_1_082112
C57BL/6 mouse bone marrow cells were stained with CD117 FITC and Alexa Fluor® 700 lineage cocktail (top plot) or Alexa Fluor® 700 isotype controls (bottom plot).
  • Lineage_Cocktail_AF700_Antibody_FC_1_082112
    C57BL/6 mouse bone marrow cells were stained with CD117 FITC and Alexa Fluor® 700 lineage cocktail (top plot) or Alexa Fluor® 700 isotype controls (bottom plot).
  • Lineage_Cocktail_AF700_Antibody_FC_2_082112
Cat # Size Price Quantity Avail. Save
133313 25 tests $250.00
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Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Alexa Fluor® 700 Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. Alexa Fluor® 700 Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

 

Materials provided:
1. Alexa Fluor® 700 anti-mCD3/ Alexa Fluor® 700 anti-mLy-6G(Ly-6C)/ Alexa Fluor® 700 anti-mCD11b/ Alexa Fluor® 700 anti-mCD45R(B220)/ Alexa Fluor® 700 anti-mTer-119
2. Alexa Fluor® 700 Rat IgG2b/Rat IgG2a isotype controls

Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
This two-reagent set contains Alexa Fluor® 700 conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)

Antigen Details

Distribution

T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes

Antigen References

1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp. Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872.

Gene ID
104231 View all products for this Gene ID 104231 View all products for this Gene ID
UniProt
View information about Lineage on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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