- Clone
- 201A (See other available formats)
- Other Names
- CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral mononuclear cells were stained with CD123 PE and CD303 (clone 201A) Alexa Fluor® 700 (top) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (bottom). Data shown was gated on lymphocyte and monocyte populations. -
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 354227 | 25 tests | $130 | ||||
| 354228 | 100 tests | $320 | ||||
CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_2629743 (BioLegend Cat. No. 354227)
AB_2629744 (BioLegend Cat. No. 354228)
Antigen Details
- Structure
- Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
- Distribution
-
Plasmacytoid dendritic cells
- Function
- Antigen capture, inhibition of IFN α/β production
- Cell Type
- Dendritic cells
- Biology Area
- Immunology, Innate Immunity, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
2. Graham LM and Brown GD. 2009. Cytokine 48:148.
3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564.
4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823. - Gene ID
- 170482 View all products for this Gene ID
- UniProt
- View information about CD303 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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