Alexa Fluor® 700 anti-human CD244 (2B4) Antibody

Pricing & Availability
Clone
C1.7 (See other available formats)
Workshop
VII 70350
Other Names
2B4, SLAMF4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
C1dot7_A700_CD244_Antibody_1_062317
Human peripheral blood lymphocytes were stained with CD8 FITC and CD244 (2B4) (clone C1.7) Alexa Fluor® 700 (top) or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom).
  • C1dot7_A700_CD244_Antibody_1_062317
    Human peripheral blood lymphocytes were stained with CD8 FITC and CD244 (2B4) (clone C1.7) Alexa Fluor® 700 (top) or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom).
  • C1dot7_A700_CD244_Antibody_2_062317
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
329525 25 tests $125
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329526 100 tests $295
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Description

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8+ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Valiante NM and Trinchieri G. 1993. J. Exp. Med. 178:1397.
  2. Chuang SS, et al. 2000. Immunology. 100:378.
  3. Hoffmann SC, et al. 2011. J. Immunol. 186:2757. PubMed
  4. Rossi LE, et al. 2012. J Leukoc Biol. 91:321. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
RRID
AB_2687331 (BioLegend Cat. No. 329525)
AB_2687332 (BioLegend Cat. No. 329526)

Antigen Details

Structure
38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution

NK, T subset, monocytes, basophils, and eosinophils

Function
Activation and inhibition of NK cell function
Ligand/Receptor
CD48
Cell Type
Basophils, Eosinophils, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Lee KM, et al. 2003. J. Immunol. 170:4881.
2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159.
3. Messmer B, et al. 2006. J. Immunol. 176:4646.
4. Boles KS, et al. 2001. Immunol. Rev. 181:234.

Gene ID
51744 View all products for this Gene ID
UniProt
View information about CD244 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 06/23/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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