Alexa Fluor® 647 anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody

Pricing & Availability
Clone
GL7 (See other available formats)
Regulatory Status
RUO
Other Names
Ly77, T and B cell activation marker
Isotype
Rat IgM, κ
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Product Citations
publications
1_GL7_AF647_GL7_Antibody_FC_1_060413
Balb/c mouse bone marrow cells were stained with anti-mouse IgD PE and anti-mouse GL7 (clone GL7) Alexa Fluor® 647 (top) or rat IgM, κ Alexa Fluor® 647 isotype control (bottom).
  • 1_GL7_AF647_GL7_Antibody_FC_1_060413
    Balb/c mouse bone marrow cells were stained with anti-mouse IgD PE and anti-mouse GL7 (clone GL7) Alexa Fluor® 647 (top) or rat IgM, κ Alexa Fluor® 647 isotype control (bottom).
  • 2_GL7_AF647_GL7_Antibody_FC_2_060413
  • GL7_A647_GL7_Antibody_1_012022
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of GL7 (clone GL7) Alexa Fluor® 647 (red), CD3 (clone 17A2) Alexa Fluor® 594 (green) and B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. The image was captured by 10X objective.
  • GL7_A647_GL7_Antibody_2_012022
    LPS-challenged C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 μg/ml of GL7 (clone GL7) Alexa Fluor® 647 (red), CD3 (clone 17A2) Alexa Fluor® 594 (green) and B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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144605 25 µg $88
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144606 100 µg $248
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Description

The GL7 antigen, also known as Ly77, is a 35 kD protein.  The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes.  It is upregulated on mouse splenocytes following activation.  It may play a role in regulation or adhesion.  GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS activated DBA/J mouse B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
  2. Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
  3. Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
  4. Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
  5. Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
Product Citations
  1. Castiblanco D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01833. PubMed
  2. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  3. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  4. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  5. Essig K et al. 2017. Immunity. 47(6):1067-1082 . PubMed
  6. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  7. Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed
  8. Ise W, et al. 2018. Immunity. 48:702. PubMed
  9. Zanotti KJ, et al. 2019. J Immunol. 202:1573. PubMed
  10. Woodruff MC et al. 2018. Cell reports. 25(2):321-327 . PubMed
  11. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  12. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  13. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  14. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  15. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  16. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  17. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  18. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
  19. Wu S, et al. 2016. Proc Natl Acad Sci U S A. 113: 9816 - 9821. PubMed
  20. Maul R, et al. 2016. J Exp Med. 213: 1675 - 1683. PubMed
  21. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  22. Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed
  23. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  24. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  25. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
RRID
AB_2562184 (BioLegend Cat. No. 144605)
AB_2562185 (BioLegend Cat. No. 144606)

Antigen Details

Structure
35 kD
Distribution

Germinal center B cells, activated B and T cells

Function
Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
Ligand/Receptor
Neu5Ac-recognizing lectins
Cell Type
B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.

Gene ID
NA
UniProt
View information about GL7 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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