- Clone
- AF6-88.5 (See other available formats)
- Other Names
- MHC class I
- Isotype
- Mouse (BALB/c) IgG2a, κ
- Ave. Rating
- 1 reviews
- Product Citations
- publications
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C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor® 647
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 116511 | 25 µg | $85 | ||||
| 116512 | 100 µg | $195 | ||||
The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).
Product DetailsProduct Details
- Reactivity
- Mouse b haplotype
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C57BL mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC, IF - Reported in the literature - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).
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Application References
(PubMed link indicates BioLegend citation) -
- Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
- Wall KA, et al. 1983. J. Immunol. 131:1056.
- Andersson M, et al. 1998. J. Immunol. 161:6475.
- Shao H, et al. 2005. J. Immunol. 175:1851.
- Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
- Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
- Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
- Product Citations
-
- RRID
-
AB_492918 (BioLegend Cat. No. 116511)
AB_492917 (BioLegend Cat. No. 116512)
Antigen Details
- Structure
- MHC class I
- Distribution
-
Nucleated cells of the H-2Kb haplotype
- Function
- Antigen presentation
- Ligand Receptor
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369. - Gene ID
- 14972 View all products for this Gene ID
- UniProt
- View information about H-2Kb on UniProt.org
Reviews
-
- Title
- Rating
- Date
-
04/17/2013
Related Pages & Pathways
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