Alexa Fluor® 647 anti-mouse FR4 (Folate Receptor 4) Antibody

Pricing & Availability
12A5 (See other available formats)
Other Names
Folate Receptor 4, FBP, FR δ
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and 12A5 Alexa Fluor® 647
  • 12A5_Alx647_070808.jpg
    C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and 12A5 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
125009 25 µg $125
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Folate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9).It was recently reported that FR4 is highly and constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg).The high expression of this surface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of T cells.Since FR4 is a surface marker,this antibody may be used in combination with anti-CD4 and/or CD25 to sort living T reg cells for functional studies.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Yamaguchi T et al. 1007:Immunity 27(1)145
Product Citations
  1. Inoue N, et al. 2015. Nat Commun. 6: 8858. PubMed
Publication Library
AB_1134201 (BioLegend Cat. No. 125009)

Antigen Details

28 kd protein consisting of 244 amino acids. Heavily glycosylated

Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells

Mediate calcium-independent homophlic cell-cell adhesion
Folic acid and its derivative
Cell Type
Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Antigen References

1. Yamaguchi T et al 2007. Immunity 27:145
2. Spiegelstein O et al. 2000 Gene 258:117

Gene ID
64931 View all products for this Gene ID
View information about FR4 on

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Forgot your password? Reset Password
Request an Account