Alexa Fluor® 647 anti-mouse CD272 (BTLA) Antibody

Pricing & Availability
8F4 (See other available formats)
Regulatory Status
Other Names
BTLA, B and T lymphocyte attenuator
Mouse IgG1, κ
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Product Citations
C57BL/6 splenocytes stained with 8F4 Alexa Fluor® 647 and CD3e (145-2C11) PE
  • A_8F4_A647_102209
    C57BL/6 splenocytes stained with 8F4 Alexa Fluor® 647 and CD3e (145-2C11) PE
  • B_8F4_A647_102209
    C57BL/6 splenocytes stained with Alexa Fluor® 647 mouse IgG1 isotype control and CD3e (145-2C11) PE
Compare all formats See Alexa Fluor® 647 spectral data
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134808 100 µg $323
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B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, reduced effectiveness of T regulatory cells. BTLA takes an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effector and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4. But further studies are needed to confirm. The existence of three distinct BTLA alleles was reported. The BTLA antibody reacts with mouse BTLA from both BALb/c and C57BL/6 strains.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
C57BL/6 BTLA Ig domain protein in CFA
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hurchla MA, et al. 2005. J. Immunol. 174:3377
Product Citations
  1. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
AB_2067836 (BioLegend Cat. No. 134808)

Antigen Details

An Ig superfamily coinhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.
BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4+T cells, lower express on CD8+ T cells, macrophages, dendritic cells, NKT cells, and NK cells.
BTLA functions as a negative regulator of T cell activation and proliferation. It attenuates B cell proliferation upon associating with its known ligand, Herpes Virus Entry Mediator (HVEM).
Herpes Virus Entry Mediator (HVEM)
Cell Type
Lymphocytes, B cells, T cells, Dendritic cells, NKT cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Liu X, et al. 2009. J. Immunol. 182:4516
2. Miller ML, et al. 2009. J. Immunol. 183:32
3. Sun Y, et al. 2009. J. Immunol. 183:1946
4. Vendel AC, et al. 2009. J. Immunol. 182:1509
5. Watanabe N, et al. 2003. Nat. Immunol. 4(7):670
6. Sedy JR, et al. 2005. Nat Immunol. 6(1):90

Gene ID
208154 View all products for this Gene ID
View information about CD272 on

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Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-mouse CD272 (BTLA)

  • Alexa Fluor® 647 anti-mouse CD272 (BTLA)

  • APC anti-mouse CD272 (BTLA)


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