Alexa Fluor® 647 anti-mouse CD169 (Siglec-1) Antibody

Pricing & Availability
Clone
3D6.112 (See other available formats)
Other Names
Sialic acid binding Ig-like lectin 1 (Siglec-1), Sialoadhesin (Sn)
Isotype
Rat IgG2a, κ
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Product Citations
publications
A_3D6.112_A647_CD169_Antibody_FC_1_060914.jpg
C57BL/6 mouse bone marrow cells were stained with F4/80 PE, Ly-6G FITC and CD169 (clone 3D6.112) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom). Data were analyzed by gating on Ly-6Gdim/neg cell population.
  • A_3D6.112_A647_CD169_Antibody_FC_1_060914.jpg
    C57BL/6 mouse bone marrow cells were stained with F4/80 PE, Ly-6G FITC and CD169 (clone 3D6.112) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom). Data were analyzed by gating on Ly-6Gdim/neg cell population.
  • B_3D6.112_A647_CD169_Antibody_FC_2_060914
  • C_3D6.112_A647_CD169_IHCF_120817
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 2.5 µg/ml of CD169 (clone 3D6.112) Alexa Fluor® 647 (red), 2.5 µg/ml of B220 (clone RA3‐6B2) Alexa Fluor® 488 (blue), and 2.5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 594 (green) overnight at 4˚C. The image was captured by 10X objective.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
142407 25 µg $145.00
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142408 100 µg $325.00
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Description

CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains). CD169 binds to sialic acids, which can be found on PSGL-1, CD43, CD206, and CD227. By its affinity to α2, 3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells. CD169 was initially identified as a sialic acid-dependent sheep erythrocyte receptor (SER) on resident bone marrow cells of mice. It has been identified as highly expressed on resident bone marrow macrophages which plays an important role in retention of stem cells in mesenchymal stem cell niche. It is also found on some specific subsets of tissue macrophages in spleen, lymph nodes, bone marrow, liver, colon, lungs, and cancer cells. Evidence suggest that CD169-positive macrophages serve as lymph node-resident APCs to dominate early activation of tumor antigen-specific CD8+ T cells and invariant NK cell.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Native Sialoadhesin from spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  For immunohistochemisty on frozen tissue sections, a concentration range of 1-5 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F)
  2. Chtanova T, et al. 2008. Immunity 29:487.
  3. Klass M, et al. 2012. J. Immunol. 189:2414. PubMed
  4. Le Gars M, et al. 2016. J Immunol. 197: 3225 - 3232. PubMed
Publication Library
RRID
AB_2563620 (BioLegend Cat. No. 142407)
AB_2563621 (BioLegend Cat. No. 142408)

Antigen Details

Structure
Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution

Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.

Function
Adhesion.
Ligand Receptor
PSGL-1, CD43, CD206 and CD227.
Antigen References

1. Chow A, et al. 2011. J. Exp. Med. 208:261.
2. Asano K, et al. 2011. Immunity 34:85.
3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
4. Varki A, et al. 2009. Glycoconj. J. 26:231.
5. Rempel H, et al. 2008. PLoS One 3:e1967.
6. Crocker PR, et al. 2001. Trends Immunol. 22:337.
7. Hartnell A, et al. 2001. Blood 97:288.
8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.

Gene ID
20612 View all products for this Gene ID
UniProt
View information about CD169 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/27/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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