Alexa Fluor® 647 anti-mouse CD106 Antibody

Pricing & Availability
Clone
429 (MVCAM.A) (See other available formats)
Other Names
VCAM-1, INCAM-110
Isotype
Rat IgG2a, κ
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Product Citations
publications
429(MVCAM.A)_AF647_CD106_Antibody_1_FC_011108
C57BL/6 bone marrow myeloid cells stained with 429 Alexa Fluor® 647
  • 429(MVCAM.A)_AF647_CD106_Antibody_1_FC_011108
    C57BL/6 bone marrow myeloid cells stained with 429 Alexa Fluor® 647
  • 429(MVCAM.A)_AF647_CD106_Antibody_2_IHCF_092016
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 647 (green) and 10 µg/ml of CD117 (clone 2B8) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 429(MVCAM.A)_AF647_CD106_Antibody_3_IHCF_032719
    Dissected C57/B6 mouse testis was immersed in 4% paraformaldehyde (PFA) overnight followed by 30% sucrose immersion overnight and frozen in OCT. Frozen section was blocked with 5% FBS and 5% mouse serum for 30 minutes at room temperature. Then the tissue section was stained with 5 µg/mL of anti-mouse CD147 (clone OX-114) Alexa Fluor® 594 (green) and CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 647 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 429(MVCAM.A)_AF647_CD106_Antibody_3_IHCF_032719
    Formalin-fixed, 300 micron-thick mouse testis section was blocked, permeabilized and stained overnight with CD106 (clone 429, MCVAM.A) Alexa Fluor® 647 (red), and CD147 (clone OX-114) Alexa Fluor® 488 (green) both at 5 µg/mL, optically cleared, and analyzed at 225 µm imaging depth on a confocal microscope. Watch the video.
See Alexa Fluor® 647 spectral data See high resolution IHC-F data...
Cat # Size Price Quantity Avail. Save
105711 25 µg $85
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105712 100 µg $195
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Description

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α41 integrin) and LPAM-1 (α47 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse preadipose cell line PA6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3-D IHC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 2.5 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, and immunoprecipitation1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
  2. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  3. Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
  4. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  5. Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
  6. Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
  7. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  8. Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
  9. Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
  10. Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
Product Citations
  1. Nakagawa R, et al. 2013. J Immunol. 190:3309. PubMed
  2. Adamiak M, et al. 2015. Leukemia. . PubMed
  3. Armaka M, et al. 2018. Nat Commun. 9:618. PubMed
  4. Onder L, et al. 2017. Immunity. 47:80. PubMed
Publication Library
RRID
AB_493430 (BioLegend Cat. No. 105711)
AB_493429 (BioLegend Cat. No. 105712)

Antigen Details

Structure
Ig superfamily, 47 kD
Distribution

Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells

Function
Adhesion
Ligand/Receptor
VLA-4 (α41 integrin) and LPAM-1 (α47 integrin)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.

Gene ID
22329 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 05/08/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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