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Alexa Fluor® 647 anti-IDO1 Antibody

Pricing & Availability
Clone
2E2/IDO1 (See other available formats)
Regulatory Status
RUO
Other Names
Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2E2-IDO1_A647_IDO1_Antibody_ICFC_1_121214
Mouse IDO1 transfected 293 cells were treated overnight (top) or untreated (bottom) with doxycycline and then were intracellularly stained with IDO1 (clone 2E2/IDO1) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 (open histogram) isotype control.
  • 2E2-IDO1_A647_IDO1_Antibody_ICFC_1_121214
    Mouse IDO1 transfected 293 cells were treated overnight (top) or untreated (bottom) with doxycycline and then were intracellularly stained with IDO1 (clone 2E2/IDO1) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 (open histogram) isotype control.
  • 2E2-IDO1_A647_IDO1_Antibody_ICFC_2_121214
Compare all formats See Alexa Fluor® 647 spectral data
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654003 25 µg $159
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654004 100 µg $370
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Description

IDO1 is also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenase. IDO is a ubiquitously expressed cytoplasmic protein with a predicted molecular weight of approximately 45 kD. Indoleamine 2,3-dioxygenase (IDO) is one the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, kidney, and placenta.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant mouse IDO1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 2E2/IDO1 reacts with mouse IDO1. It does not appear to react with mouse IDO2.

Clone 2E2/IDO1 is the same clone as anti-IDO1 clone 2E2.6.

Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  3. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  4. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  5. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
RRID
AB_2564584 (BioLegend Cat. No. 654003)
AB_2564585 (BioLegend Cat. No. 654004)

Antigen Details

Structure
403 amino acids with a predicted molecular weight of 45 kD.
Distribution

Cytoplasm

Function
Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens.
Interaction
This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.
Biology Area
Cell Biology, Immunology
Antigen References

1. Habara-Ohkubo A, et al. 1991. Gene 105:221.
2. Munn DH, et al. 2002. Science 297:1867.
3. Frumento G, et al. 2002. J. Exp. Med. 196:459.
4. Muller AJ, et al. 2005. Nature Med. 11:312.
5. Lob S, et al. 2009. Nature Rev. Cancer 9:445.

Gene ID
15930 View all products for this Gene ID
UniProt
View information about IDO1 on UniProt.org
Go To Top Version: 1    Revision Date: 12/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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