- 259D (See other available formats)
- Other Names
- Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
- Mouse IgG1, κ
- Ave. Rating
- 2 reviews
- Product Citations
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FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 259D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.Product Details
- Human, Cross-Reactivity: Cynomolgus, Rhesus, Baboon, Chimpanzee
- Antibody Type
- Host Species
- Full-length FOXP3 protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1, and immunohistochemical staining1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The 259D antibody gives strong positivity on paraffin and frozen sections and the antibody stains some epithelial cells. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
(PubMed link indicates BioLegend citation)
- Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
- Yang ZZ, et al. 2006. Blood 107:3639. PubMed
- Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. PubMed
- Groh V, et al. 2006. Nature Immunology 7:755. PubMed
- Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656.PubMEd
- Long SA, et al. 2008. J Autoimmun. 30:293. PubMed
- Gong G, et al. 2009. Blood 113:837. PubMed
- Long SA, et al. 2009. Eur J. Immunol. 39:612. PubMed
- Long SA, et al. 2010. Diabetes. 59:407. PubMed
- Ferraro A, et al. 2014. PNAS. 111:1111. PubMed
- Vudattu NK, et al. 2014. J Immunol. 193:587. PubMed
- Dupont G, et al. 2014. Cytokine. 69:146. PubMed
- Product Citations
AB_492985 (BioLegend Cat. No. 320213)
AB_492984 (BioLegend Cat. No. 320214)
- Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Nuclear; expressed in T regulatory cells
- Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
- Interacts with DNA
- Cell Type
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Hori S, et al. 2003. Science 299:1057.
- FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
- Gene ID
- 50943 View all products for this Gene ID
- View information about FOXP3 on UniProt.org
- Can FOXP3 be costained with cytokines?
The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.
- Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?
It is not recommended. It is best to use PBMCs for this testing.
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