Alexa Fluor® 647 anti-GFP Antibody

Pricing & Availability
FM264G (See other available formats)
Regulatory Status
Other Names
Green Fluorescent Protein
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
GFP transfected CHO cells intracellularly stained with FM264G Alexa Fluor® 647 or isotype control rat IgG2a Alexa Fluor® 647
  • FM264G_A647_121508.jpg
    GFP transfected CHO cells intracellularly stained with FM264G Alexa Fluor® 647 or isotype control rat IgG2a Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
338005 25 tests $125.00
Check Availability

Need larger quantities of this item?
Request Bulk Quote
338006 100 tests $265.00
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea Victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Product Details
Technical data sheet

Product Details

GFP-tagged proteins
Antibody Type
Host Species
TLR9-GFP transfected cell line
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry1.

Application References

(PubMed link indicates BioLegend citation)
  1. Stephen LA, et al. 2018. Dev. Cell. 47(1):122-132.e4. PubMed (ICC)
Product Citations
  1. Sperber HS, et al. 2019. Sci Rep. 9:707. PubMed
  2. Abels ER et al. 2019. Cell Rep. 28(12):3105-3119 . PubMed
  3. Barrera-Chimal J, et al. 2013. Nephrol Dial Transplant. 29:301. PubMed
  4. Coomes S, et al. 2015. PLoS One. 11: 1004994. PubMed
  5. Stergiopoulos A, Politis P 2016. Sci Rep. 6:29859. PubMed
  6. Silvester E, et al. 2021. Cell. 184(4):1110-1121.e16. PubMed
AB_1279413 (BioLegend Cat. No. 338005)
AB_1279411 (BioLegend Cat. No. 338006)

Antigen Details

Biology Area
Cell Biology, Immunology
Antigen References

1. Ishikura H, et al. 2004. Anticancer Res. 24:719.
2. Rizzuto R, et al. 1996. Curr. Biol. 6:183.
3. Chalfie M, et al. 1994. Science 263:802.

Gene ID
View information about GFP on

Related FAQs

Can I use common compensation control for GFP, CFSE and FITC because they emit in the same channel?
It is not recommended even if they emit in the same channel because these are still different fluors with different brightness intensities. Individual compensation controls should be employed.
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Insert Note Here
Save Close Clear
Lab Timer
Remember me
Forgot your password? Reset Password
Request an Account