Alexa Fluor® 647 anti-GFP Antibody

Pricing & Availability
Clone
1GFP63 (See other available formats)
Other Names
Green fluorescent protein, GFP
Isotype
Mouse IgG2a
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Product Citations
publications
1GFP63_A647_GFP_Antibody_053018
HeLa cells transiently transfected with GFP fused protein (C-F) or non-transfected (A and B) were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:500 (1 µg/ml, B and F) and 1: 2000 (0.25 µg/ml, D) Alexa Fluor® 647 anti-GFP Antibody (red). The images were also acquired from the same cells under GFP channel (Green, A, C and E). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective.
  • 1GFP63_A647_GFP_Antibody_053018
    HeLa cells transiently transfected with GFP fused protein (C-F) or non-transfected (A and B) were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:500 (1 µg/ml, B and F) and 1: 2000 (0.25 µg/ml, D) Alexa Fluor® 647 anti-GFP Antibody (red). The images were also acquired from the same cells under GFP channel (Green, A, C and E). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
668211 25 µg $85
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668212 100 µg $195
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Description

Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins. This makes GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Product Details
Technical data sheet

Product Details

Reactivity
Aequorea victoria
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 0.25 - 1.0 µg/mL (1:500 - 1:2000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_2734529 (BioLegend Cat. No. 668211)
AB_2734530 (BioLegend Cat. No. 668212)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Ishikura H, et al. 2004. Anticancer Res. 24:719.
2. Rizzuto R, et al. 1996. Curr. Biol. 6:183.
3. Chalfie M, et al. 1994. Science 263:802.

Gene ID
NA
UniProt
View information about GFP on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/13/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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