Alexa Fluor® 647 anti-GFAP Antibody

Pricing & Availability
Clone
SMI 25 (See other available formats)
Other Names
Glial fibrillary acidic protein
Isotype
Mouse IgG2b, κ
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Product Citations
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SMI-25_A647_GFAP_IHCP_Antibody_1_061518
IHC staining of Alexa Fluor® 647 anti-GFAP antibody (clone SMI 25) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 10 µg/ml overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-25_A647_GFAP_IHCP_Antibody_1_061518
    IHC staining of Alexa Fluor® 647 anti-GFAP antibody (clone SMI 25) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 10 µg/ml overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-25_A647_GFAP_IHCP_Antibody_2_061518
    IHC staining of Alexa Fluor® 647 anti-GFAP antibody (clone SMI 25) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-25_A647_GFAP_IHCP_Antibody_3_061518
    IHC staining of Alexa Fluor® 647 anti-GFAP antibody (clone SMI 25) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 10 µg/ml overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
837511 25 µg $110
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837512 100 µg $275
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Description

Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts, Leydig cells of the testis, keratinocytes, osteocytes and chondrocytes and stellate cells of the pancreas and liver. GFAP is a type III IF protein that is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells.

Type III intermediate filaments are highly conserved and contain three domains, named the head, rod and tail domains. This rod domain coils around that of another filament to form a dimer, with the N-terminal and C-terminal of each filament aligned. Type III filaments such as GFAP are capable of forming both homodimers and heterodimers; GFAP can polymerize with other type III proteins or with neurofilament protein (NF-L). Interestingly, GFAP and other type III IF proteins cannot assemble with keratins, the type I and II intermediate filaments: in cells that express both proteins, two separate intermediate filament networks form.

To form networks, the initial GFAP dimers combine to make staggered tetramers, which are the basic subunits of an intermediate filament. The non-helical head and tail domains are necessary for filament formation. The head and tail regions have greater variability of sequence and structure. In spite of this increased variability, the head of GFAP contains two conserved arginines and an aromatic residue that are required for proper assembly.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_2734610 (BioLegend Cat. No. 837511)
AB_2734611 (BioLegend Cat. No. 837512)

Antigen Details

Structure
GFAP is a 432 amino acid protein with a molecular mass of approximately 50 kD.
Distribution

Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.

Cellular distribution: cytoskeleton and cytosol

Function
GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Khakh BS, Sofroniew MV. 2015. Nat. Neurosci. 18:942-52. PubMed

Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/21/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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