Alexa Fluor® 647 anti-GFAP Antibody

Pricing & Availability
Clone
2E1.E9 (See other available formats)
Other Names
Glial fibrillary acid protein
Isotype
Mouse IgG2b
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2E1.E9_AF647_GFAP_Antibody_1_072915
Day-three cultured postnatal C57BL/6 mouse brain cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2.5 µg/ml of anti-GFAP (clone 2E1.E9) Alexa Fluor® 647 in a blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 2E1.E9_AF647_GFAP_Antibody_1_072915
    Day-three cultured postnatal C57BL/6 mouse brain cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2.5 µg/ml of anti-GFAP (clone 2E1.E9) Alexa Fluor® 647 in a blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 2E1dotE9_A647_GFAP_Antibody_IHC_2_091117
    Human paraffin-embedded cerebellum tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0M, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-GFAP Antibody (Clone 2E1.E9, red) and Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) Antibody (Clone AA10, green) antibody overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 2E1dotE9_A647_GFAP_Antibody_IHC_3_091117
    Human paraffin-embedded cerebellum tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0M, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-GFAP Antibody (Clone 2E1.E9, red) and Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) Antibody (Clone AA10, magenta) antibody overnight at 4°C. Nuclei were counterstained with DAPI (green). The image was scanned with a 10X objective and stitched with MetaMorph® software.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
644706 100 µg $275.00
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Description

GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
Bovine spinal cord homogenate
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IF - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 0.1 - 10 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.


* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221.
  2. Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
Publication Library
RRID
AB_2566110 (BioLegend Cat. No. 644706)

Antigen Details

Structure
Around 50 kD
Distribution

Only expressed in astrocytes in the central nervous system.

Function
Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/30/2015

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