Alexa Fluor® 594 anti-Tau, 1-100 Antibody

Pricing & Availability
Clone
43D (See other available formats)
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1
Isotype
Mouse IgG1
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Product Citations
publications
43D_A594_Tau_1-100_Antibody_1_092117
Immunofluorescence staining of Alexa Fluor® 594 anti-Tau, 1-100 antibody (clone 43D) on formalin-fixed, paraffin-embedded human Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.), the tissue was incubated with the Alexa Fluor® 594-conjugated antibody at 1 µg/mL overnight at 4°C. Nuclei were stained with DAPI. Images captured were with a 40x objective.
  • 43D_A594_Tau_1-100_Antibody_1_092117
    Immunofluorescence staining of Alexa Fluor® 594 anti-Tau, 1-100 antibody (clone 43D) on formalin-fixed, paraffin-embedded human Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.), the tissue was incubated with the Alexa Fluor® 594-conjugated antibody at 1 µg/mL overnight at 4°C. Nuclei were stained with DAPI. Images captured were with a 40x objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
816605 25 µg $110
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816606 100 µg $275
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against full-length human tau protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
IHC - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1.0 -5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is reactive to amino acid residues 1-100 of human Tau. The epitope lies within amino acids 6-18. This clone is specific for all six isoforms of human tau protein.

This antibody clone has been reported for use on IHC of 4% PFA-fixed free floating sections5.

This antibody is exclusively provided by BioLegend.

RRID
AB_2716051 (BioLegend Cat. No. 816605)
AB_2716052 (BioLegend Cat. No. 816606)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References
  1. Augustinack JC, et al. 2002. Acta Neuropath. 103(1):26-35.
  2. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.
  3. Dong Y, et al. 2012. PLoS One. 7(6):e39386.
  4. Simic G, et al. 2016. Biomolecules. 6(1):6.
  5. Dai CL, et al. 2017. Alzheimers Res Ther. 9(1):1. (IHC, WB) [PubMed]
  6. Baazaoui N, et al. 2017. Alzheimers Res Ther. 9:45. ( WB) [PubMed]
  7. Dai CL, et al. 2015. J neural Transm (Vienna). 122:607.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau, 1-100 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 09/22/2017

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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