Alexa Fluor® 594 anti-Synaptotagmin-12 Antibody

Pricing & Availability
Clone
N277/7 (See other available formats)
Other Names
Synaptotagmin-12, sytXII, synaptotagmin 12, Syt12, Synaptotagmin XII, Synaptotagmin-related gene 1 protein, Srg1, Sytr1
Isotype
Mouse IgG1
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Product Citations
publications
N277slash7_A594_Synaptotagmin_Antibody_1_070218
IHC staining of Alexa Fluor® 594 anti-Synaptotagmin-12 antibody (clone N277/7) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • N277slash7_A594_Synaptotagmin_Antibody_1_070218
    IHC staining of Alexa Fluor® 594 anti-Synaptotagmin-12 antibody (clone N277/7) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • N277slash7_A594_Synaptotagmin_Antibody_2_070218
    IHC staining of Alexa Fluor® 594 anti-Synaptotagmin-12 antibody (clone N277/7) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • N277slash7_A594_Synaptotagmin_Antibody_3_070218
    IHC staining of Alexa Fluor® 594 anti-Synaptotagmin-12 antibody (clone N277/7) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
820405 25 µg $110
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820406 100 µg $180
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Description

Synaptotagmin 12 (Syt12) is regulated by protein kinase A (PKA) and is unique from other synaptotagmins in that it does not bind to Ca2+. Syt12 interaction with Syt1 prevents SNARE complex binding. Expression of Syt12 induces spontaneous neurotransmitter release, providing a mechanism for spontaneous synaptic-vesicle exocytosis that is Ca2+-independent.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Additional Product Notes

The reactivity of Alexa Fluor® 594 anti-Synaptotagmin-12 antibody was validated in FFPE mouse and rat brain tissues.

RRID
AB_2749909 (BioLegend Cat. No. 820405)
AB_2749910 (BioLegend Cat. No. 820406)

Antigen Details

Structure
Expected MW: 47 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
171180 View all products for this Gene ID
UniProt
View information about Synaptotagmin-12 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/02/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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