Alexa Fluor® 594 anti-SNAP-25 Antibody

Pricing & Availability
Clone
SMI 81 (See other available formats)
Other Names
Synaptosomal-associated protein 25, SUP, Synaptosomal-associated 25 kD Protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
SMI-81_A594_SNAP25_Antibody_1_082718
IHC staining of Alexa Fluor® 594 anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • SMI-81_A594_SNAP25_Antibody_1_082718
    IHC staining of Alexa Fluor® 594 anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • SMI-81_A594_SNAP25_Antibody_2_082718
    IHC staining of Alexa Fluor® 594 anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • SMI-81_A594_SNAP25_Antibody_3_082718
    IHC staining of Alexa Fluor® 594 anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
836309 25 µg $135
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836310 100 µg $275
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Description

Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca2+-triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length, unaltered SNAP-25.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional applications have been reported for relevant formats, including: EM2, FC1, IF3,4,7, IP5,8.

Application References

(PubMed link indicates BioLegend citation)
  1. Postupna NO, et al. 2014. Lab Invest. 94:1161. (FC)
  2. Hirano AA, et al. 2011. J. Comp. Neurol. 519:972. (WB, IHC, EM) PubMed
  3. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
  4. Rozas JL, et al. 2012. Neuron. 74:151. (IF) PubMed
  5. Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed
  6. Gu Y, et al. 2016. Proc. Natl. Acad. Sci. U. S. A. E922-31. (WB) PubMed
  7. Kabachinski G, et al. 2016. Mol. Biol. Cell. 654-68.(IF) PubMed
  8. Kunii M, et al. 2016. J. Cell Biol. 215:121-138. (IP) PubMed
RRID
AB_2750110 (BioLegend Cat. No. 836309)
AB_2750111 (BioLegend Cat. No. 836310)

Antigen Details

Structure
SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
Distribution

Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Cellular distribution: Cytosol, golgi apparatus, and cell membrane.

Function
SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
Interaction
In adult tissue, SMI 81 visualizes synapse-rich areas of brain and retina. In developing brain and cell cultures, SMI 81 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References

1. Snyder DA, et al. 2006. Cell Biochem. Biophys. 45(1):111-23.
2. Nagy G, et al. 2004. Neuron. 41(3):417–29.

Gene ID
6616 View all products for this Gene ID
UniProt
View information about SNAP-25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/27/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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