Alexa Fluor® 594 anti-rat IgG1 Antibody

Pricing & Availability
Clone
MRG1-58 (See other available formats)
Other Names
Imunoglobulin G1
Isotype
Mouse IgG
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Product Citations
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MRG1-58_A694_IgG1_Antibody_IHC_110917
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Purified CD8b.2 (clone 53-5.8) at 4°C overnight. The staining was followed by 5 µg/ml anti-Rat IgG1 (clone MRG1-58) Alexa Fluor® 594 (red) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488(green) at room temperature for 2 hours. The image was captured by 10X objective.
  • MRG1-58_A694_IgG1_Antibody_IHC_110917
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Purified CD8b.2 (clone 53-5.8) at 4°C overnight. The staining was followed by 5 µg/ml anti-Rat IgG1 (clone MRG1-58) Alexa Fluor® 594 (red) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488(green) at room temperature for 2 hours. The image was captured by 10X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
407409 25 µg $95
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407410 100 µg $185
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Description

The MRG1-58 monoclonal antibody reacts with rat immunoglobulin G1 (IgG1) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG1-58 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. 


Alexa Fluor® is a trademark of Life Technologies Corporation.

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RRID
AB_2721399 (BioLegend Cat. No. 407409)
AB_2721400 (BioLegend Cat. No. 407410)

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Go To Top Version: 0    Revision Date: 11/15/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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