Alexa Fluor® 594 anti-Rab7A Antibody

Pricing & Availability
Clone
W16034A (See other available formats)
Other Names
Rab7, Ras-Related Protein Rab-7a, Ras-Associated Protein RAB7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16034A_A594_Rab7_Antibody_ICC_122617
ICC staining of Alexa Fluor® 594 anti-Rab7A antibody (clone W16034A) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were co-stained with 5 µg/ml of Alexa Fluor® 594 anti-Rab7A antibody and 1 µg/ml of Alexa Fluor® 488 anti-α-Tubulin antibody (clone 10D8, Cat. No. 627906) for 24 hours at 4°C. Nuclei were counterstained with DAPI. The images were captured with a 60X objective. Scale bar: 50 µm.
  • W16034A_A594_Rab7_Antibody_ICC_122617
    ICC staining of Alexa Fluor® 594 anti-Rab7A antibody (clone W16034A) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were co-stained with 5 µg/ml of Alexa Fluor® 594 anti-Rab7A antibody and 1 µg/ml of Alexa Fluor® 488 anti-α-Tubulin antibody (clone 10D8, Cat. No. 627906) for 24 hours at 4°C. Nuclei were counterstained with DAPI. The images were captured with a 60X objective. Scale bar: 50 µm.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
850405 25 µg $120
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850406 100 µg $305
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Description

Rab7, a small GTPase of the Rab family, plays multiple roles including vesicular transport, endocytic trafficking, and autophagy. Rab7 is associated with both the endosome and lysosome, and it facilitates endosomal maturation, transport from the late endosome to the lysosome, and positioning of the endosome and lysosome via regulating their movement along cytoskeleton. Consistently, mutations or dysfunctions of Rab7 result in traffic disorders, which cause various diseases, such as neuropathy, cancer and lipid metabolism disease. Rab7 also plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant human Rab7A protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

 

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2721397 (BioLegend Cat. No. 850405)
AB_2721398 (BioLegend Cat. No. 850406)

Antigen Details

Structure
Rab7A is a 207 amino acid protein with a molecular mass of 23 kD.
Distribution

Tissue distribution: Rab7A is ubiquitously expressed.
Cellular distribution: Plasma membrane, cytosol, lysosome, endosome, and Golgi apparatus.

Function
Rab7A is involved in the endocytic pathway and autophagy.
Interaction
Rab7A interacts with RILP, Rab1A, Rab11A, and UBC.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References

1. Guerra F, Bucci C. 2016. Cells. 5(3).

Gene ID
7879 View all products for this Gene ID
UniProt
View information about Rab7A on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 12/28/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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