Alexa Fluor® 594 anti-human CD66d/e Antibody

Pricing & Availability
Clone
308/3-3 (See other available formats)
Other Names
CEACAM3, CGM1, CEACAM5, CEA
Isotype
Mouse IgG1, κ
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Product Citations
publications
308slash3-3_A594A_CD66d-e_Antibody_IHC-P_1_042018
Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10mM, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes , permeabilized with PBS with 0.5% Triton X-100 for 15 mintues and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-Vimentin (clone O91D3) antibody (green) and 10µg/mL of Alexa Fluor® 594 anti-CD66d/e (clone 308/3-3) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 308slash3-3_A594A_CD66d-e_Antibody_IHC-P_1_042018
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10mM, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes , permeabilized with PBS with 0.5% Triton X-100 for 15 mintues and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-Vimentin (clone O91D3) antibody (green) and 10µg/mL of Alexa Fluor® 594 anti-CD66d/e (clone 308/3-3) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 308slash3-3_A594A_CD66d-e_Antibody_IHC-P_1_042018
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10mM, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes , permeabilized with PBS with 0.5% Triton X-100 for 15 mintues and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-Vimentin (clone O91D3) antibody (green) and 10µg/mL of Alexa Fluor® 594 anti-CD66d/e (clone 308/3-3) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
392803 25 µg $135
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392804 100 µg $325
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Description

The 308/3-3 mAb reacts with an antigen epitope shared by CD66d and e. CD66d/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66d is a major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including NeisseriaMoraxella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant soluble human CEACAM3-Fc produced in HEK293 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2734446 (BioLegend Cat. No. 392803)
AB_2734447 (BioLegend Cat. No. 392804)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Interaction
CEACAM1, 5,6, SRC and ZPLD1.
Ligand/Receptor
CD66d: CEACAM1, SRC.
CD66e: CEACAM1, 5, 6.
Cell Type
Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Chen T, et al. 2001. J Biol Chem. 276:17413
  2. Wang Z, et al. 2016. Mol Clin Oncol. 5:731
  3. Swords DS, et al. 2016. Onco Targets Ther. 9:7459
Gene ID
1084 View all products for this Gene ID 1048 View all products for this Gene ID
UniProt
View information about CD66d/e on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/10/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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