Alexa Fluor® 594 anti-human Cadherin 11 Antibody

Pricing & Availability
Clone
16G5 (See other available formats)
Other Names
OSF-4, Osteoblast cadherin, OB-cadherin, CDH11, CDHOB, CAD11
Isotype
Mouse IgG1, κ
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Product Citations
publications
16G5_A594_Cadherin11_Antibody_1_051019
H460 Human lung Carcinoma cells were fixed with 1% paraformaldehyde (PFA), then blocked for 30 minutes with 5% FBS at room temperature. Then the cells were stained with 1.25 µg/mL anti-human Cadherin 11 (clone 16G5) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DRAQ7™ for 15 minutes (blue). The image was captured with a 40x objective.
  • 16G5_A594_Cadherin11_Antibody_1_051019
    H460 Human lung Carcinoma cells were fixed with 1% paraformaldehyde (PFA), then blocked for 30 minutes with 5% FBS at room temperature. Then the cells were stained with 1.25 µg/mL anti-human Cadherin 11 (clone 16G5) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DRAQ7™ for 15 minutes (blue). The image was captured with a 40x objective.
  • 16G5_A594_Cadherin11_Antibody_2_051019
    Human paraffin-embedded ovary tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, permeabilized with 0.5% Tween20 in PBS and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluro® 594 anti-human Cadherin 11 (clone 16G5) antibody (red) and Brilliant Violet 421™ anti-Nestin (clone 10C2) antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
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368710 100 µg $315
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Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 11 is a type I transmembrane glycoprotein. It is a type II (atypical) cadherin, based on its lack of a HAV cell adhesion recognition sequence that is specific to type I cadherins. The expression of this particular cadherin in osteoblastic cell lines along with its upregulation during differentiation suggests a specific function in bone development and maintenance. This cadherin is also expressed in some cancer cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein containing the extracellular cadherin domain 1 of Cadherin 11 fused to maltose binding protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

RRID
AB_2571948 (BioLegend Cat. No. 368710)

Antigen Details

Structure
Type I single pass transmembrane glycoprotein.
Distribution

Osteoblasts, neuroblasts, and some cancer cells.

Function
Cell adhesion.
Interaction
Cadherin 2 (CDH2).
Ligand/Receptor
Calcium.
Cell Type
Osteoclasts
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References

1. Wheelock MJ, Johnson KR. 2003. Annu. Rev. Cell Dev. Biol. 19:207-35.
2. Christofori G, et al. 2003. EMBO. J. 22:2318.
3. Okazaki M, et al. 1994. J. Biol. Chem. 269:12092.

Gene ID
1009 View all products for this Gene ID
UniProt
View information about Cadherin 11 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/18/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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