Alexa Fluor® 594 anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# A594-101L)

Pricing & Availability
Clone
16B12 (See other available formats)
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# A594-101L
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
16B12_AF594_HA.11_Antibody_IF_021715
Staining of HA.11 Clone 16B12 Monoclonal Antibody, Alexa Fluor Labeled, on CHO clone 6 cells using MeOH fixation at 1:1000. Cells were counterstained with DAPI (blue).
  • 16B12_AF594_HA.11_Antibody_IF_021715
    Staining of HA.11 Clone 16B12 Monoclonal Antibody, Alexa Fluor Labeled, on CHO clone 6 cells using MeOH fixation at 1:1000. Cells were counterstained with DAPI (blue).
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
901511 100 µl $366
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA. 

Product Details
Technical data sheet

Product Details

Reactivity
YPYDVPDYA Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide, CYPYDVPDYASL.
Formulation
Phosphate-buffered solution + 5mM Sodium Azide + 0.1% BSA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
Store at 2°C-8°C. Freezing temperatures will denature the product.
Application

ICC, IF

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry.

The optimal working dilution should be determined for each specific assay condition.
IF: 1:1,000
 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective for use in immunofluorescence (IF).

This antibody was subsequently purified via a protein-G column and conjugated to Alexa Fluor® 594. Alexa Fluor® 594 is an excellent alternative to the Texas Red dye.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. HA.11 recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
  1. San-Segundo PA, Roeder GS. 2000. Mol Biol Cell. 11:3601.
  2. Kolodziej P, Young R. 1991. Meth Enzymol. 194:508.
  3. Field J, et al. 1988. Mol Cell Biol. 8:2159.
  4. Majeed SR, et al. 2014. Nat Commun. 23:5891. (IF) PubMed
  5. von Meyenn F, et al. 2013. Cell Metab.17:436. (IF)
Product Citations
  1. Majeed S, et al. 2014. Nat Commun. 5:3891. PubMed
  2. Einarsson E, et al. 2016. PLoS Negl Trop Dis. 10: 0004571. PubMed
Publication Library
RRID
AB_2565073 (BioLegend Cat. No. 901511)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 07/26/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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