- DA-7 (See other available formats)
- Other Names
- Keratin 18, Keratin type I cytoskeletal 18
- Mouse IgG1, κ
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Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein with a molecular weight of approximately 48 kD. Cytokeratin 18 is a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8 and is widely expressed in simple epithelial tissues in adults, where it is found in the cytoplasm, nucleus, and nucleolus. Cytokeratin 18 is associated with the cytoskeleton and mutations in this protein have been associated with liver disease after environmental insult. Cytokeratin 18 can be modified by glycosylation, phosphorylation (Ser34 and Ser53), acetylation (Ser2), and proteolytic cleavage at amino acids 238 and 397 by caspase family members. The cytokeratin 18 protein interacts with a number of proteins including caspase 3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, and usherin, among others.Product Details
- Human breast carcinoma cell line PMC-42
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IF - Quality tested
IHC-P - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1-5 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Application Notes
The DA-7 monoclonal antibody recognizes human cytokeratin 18 and is useful for Western blotting. Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of paraffin-embedded sections, immunocytochemistry, and ELISA.
(PubMed link indicates BioLegend citation)
- Taylor-Papadimitriou J, et al. 1989. J. Cell Sci. 94:403.
- Lauerova L, et al. 1988. Hybridoma 7:495.
AB_2563649 (BioLegend Cat. No. 628406)
- Type I intermediate filament protein, contains three coiled-coil domains, approximately 48 kD. Heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8.
Widely expressed in simple epithelial tissues in adults. Can be found in the cytoplasm, nucleus, and nucleolus
- Intermediate filament protein involved with the cytoskeleton. Mutations in cytokeratin 18 may predispose to liver disease upon exposure to some viruses or hepatoxins
- Interacts with a number of proteins including caspase3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, usherin
- Phosphorylation (Ser34, Ser53), glycosylation, acetylation (Ser2). Can also be cleaved at amino acid 238 and 397 by caspase family members
- Antigen References
1. Ku N, et al. 2003. Proc. Natl. Acad. Sci. 100:6063.
2. Kulesh DA and Oshima RG. 1989. Genomics 4:339.
3. Steinart PM and Roop DR. 1988. Ann. Rev. Biochem. 57:593.
- Gene ID
- 3875 View all products for this Gene ID
- View information about Cytokeratin 18 on UniProt.org
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