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Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.Product Details
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 555.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IF - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 2.5-5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 580 nm.
Alexa Fluor® is a trademark of Life Technologies Corporation.
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- Application Notes
This conjugated goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
(PubMed link indicates BioLegend citation)
- Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
- Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
AB_2563180 (BioLegend Cat. No. 405420)
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