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Alexa Fluor® 555 Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Isotype
Goat Polyclonal IgG
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Poly4054_AF594_IgG_Antibody_IF_033114
NIH/3T3 mouse fibroblast cell line was fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/mL purified anti-mouse/human CD44 (clone IM7) at room temperature for three hours, followed by 2.5 µg/mL anti-rat IgG (clone Poly4054) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). Image was captured with a 20X objective.
  • Poly4054_AF594_IgG_Antibody_IF_033114
    NIH/3T3 mouse fibroblast cell line was fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/mL purified anti-mouse/human CD44 (clone IM7) at room temperature for three hours, followed by 2.5 µg/mL anti-rat IgG (clone Poly4054) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). Image was captured with a 20X objective.
See Alexa Fluor® 555 spectral data
Cat # Size Price Quantity Avail. Save
405420 100 µg $160.00
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 555.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IF - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 2.5-5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 580 nm.


Alexa Fluor® is a trademark of Life Technologies Corporation.

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Application Notes

This conjugated goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
Publication Library
RRID
AB_2563180 (BioLegend Cat. No. 405420)

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Go To Top Version: 3    Revision Date: 02/08/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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