Alexa Fluor® 488 anti-TIF1β (KAP-1, TRIM28) Antibody

Pricing & Availability
Clone
20A1 (See other available formats)
Other Names
Transcription intermediary factor 1-beta, KRAB-associated protein, TRIM28, KAP-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
20A1_A488_TIF1beta_Antibody_1_062519
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1.25 µg/ml anti-TIF1β (clone 20A1) Alexa Fluor® 488 (green) in blocking buffer, overnight at 4°C, and followed by Phalloidin 594 (red) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 20A1_A488_TIF1beta_Antibody_1_062519
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1.25 µg/ml anti-TIF1β (clone 20A1) Alexa Fluor® 488 (green) in blocking buffer, overnight at 4°C, and followed by Phalloidin 594 (red) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
619306 100 µg $295
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Description

TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1, Western blotting1 and chromatin immunoprecipitation1.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

This clone may produce increased background by western blot when used at concentrations exceeding 0.1 μg/mL.

Application References

(PubMed link indicates BioLegend citation)
  1. Chang CW, et al. 2008 BMC Mol. Biol. 9:61. (WB, IF, ChIP)
RRID
AB_2566440 (BioLegend Cat. No. 619306)

Antigen Details

Structure
Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD
Distribution

Nuclear, associates with specific chromatin regions

Function
Forms a complex with KRAB-domain transcription factor and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366.
2. Schultz DC, et al. 2002. Genes Dev. 16:919.
3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859.
4. Friedman JR, et al. 1996. Genes Dev. 10:2067.
5. Messerschmidt DM, et al. 2012. Science 335:1499.
6. Chikuma S, et al. 2012. Nat. Immunol. 13:596.

 

Gene ID
10155 View all products for this Gene ID
UniProt
View information about TIF1β on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 12/16/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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