Alexa Fluor® 488 anti-SOX2 Antibody

Pricing & Availability
Clone
14A6A34 (See other available formats)
Other Names
SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3
Isotype
Mouse IgG1, κ
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Product Citations
publications
1-14A6A34_AF488_SOX2_Antibody_FC_071014
Human embryonic carcinoma NCCIT cells were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with SOX2 (clone 14A6A34) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
  • 1-14A6A34_AF488_SOX2_Antibody_FC_071014
    Human embryonic carcinoma NCCIT cells were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with SOX2 (clone 14A6A34) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
  • 2-14A6A36_A488_SOX2_Antibody_ICC_052419
    NCCIT cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes at 37°C, permeabilized with 0.5% Triton X-100 at room temperature for 10 minutes and blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS). Then the cells were stained with anti-human SOX2 (clone 14A6A34) Alexa Fluor® 488 (green) overnight, followed by Phalloidin Alexa Fluor® 594 (red) for 20 minutes at 4°C. The nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
656109 25 µg $95
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656110 100 µg $225
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Description

SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human SOX recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. Alexandrova S, et al. 2016. Development. 143: 24 - 34. PubMed
  2. Ceholski D, et al. 2017. Stem Cell Res. 10.1016/j.scr.2017.06.015. PubMed
  3. Lin JR 2018. eLife. 7: e31657. PubMed
Publication Library
RRID
AB_2563956 (BioLegend Cat. No. 656109)
AB_2563957 (BioLegend Cat. No. 656110)

Antigen Details

Structure
317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding
Distribution

Nucleus

Function
Transcription factor that regulates the expression of the genes involved in embryonic development
Interaction
Interacts with FGFR1, SOX3, and ZSCAN10
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Antigen References

1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228.
2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725.
3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772.
4. Jeong CH, et al. 2010. Stem Cells 28:2141.
5. Xiang R, et al. 2011. Br. J. Cancer 104:1410.
6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426.

Gene ID
6657 View all products for this Gene ID
UniProt
View information about SOX2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/05/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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