Alexa Fluor 488 anti-rat CD45RA Antibody anti-CD45RA

Pricing & Availability

Clone: OX-33 (See other available formats)
Regulatory Status: RUO
Other Names: Leukocyte common antigen, high molecular weight form
Isotype: Mouse IgG1, κ
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Product Citations: publications

OX-33_Alx488_021308 — LOU rat splenocytes stained with OX-33 Alexa Fluor® 488

Compare all formats See Alexa Fluor® 488 spectral data

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202310

100 µg

$229

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Description

CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Leukocyte common antigen purified from rat splenocytes.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections¹.

Application References (PubMed link indicates BioLegend citation)

Smith T, et al. 2000. Nature Med. 6:62. (IHC)

RRID

AB_572043 (BioLegend Cat. No. 202310)

Antigen Details

Structure: Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD
Distribution: Primarily on B lymphocytes
Function: B cell receptor signal transduction
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.
Gene ID: 24699 View all products for this Gene ID
UniProt: View information about CD45RA on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Stem Cells and Development

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45RA Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-rat CD45RA	OX-33	FC,IHC-F
Biotin anti-rat CD45RA	OX-33	FC
FITC anti-rat CD45RA	OX-33	FC
PE anti-rat CD45RA	OX-33	FC
Alexa Fluor® 488 anti-rat CD45RA	OX-33	FC
APC anti-rat CD45RA	OX-33	FC
PerCP/Cyanine5.5 anti-rat CD45RA	OX-33	FC
PE/Cyanine7 anti-rat CD45RA	OX-33	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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